POLR2J Protein (His tag)
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- Target See all POLR2J Proteins
- POLR2J (Polymerase (RNA) II (DNA Directed) Polypeptide J, 13.3kDa (POLR2J))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This POLR2J protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Purification
- purified by chromatography
- Purity
- > 90 % by SDS - PAGE
- Top Product
- Discover our top product POLR2J Protein
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Polymerase (RNA) II (DNA Directed) Polypeptide J, 13.3kDa (POLR2J) (AA 1-117) protein (GST tag)
POLR2J Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Polymerase (RNA) II (DNA Directed) Polypeptide J, 13.3kDa (POLR2J) (AA 1-117) protein (Strep Tag)Crystallography grade POLR2J Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB
Polymerase (RNA) II (DNA Directed) Polypeptide J, 13.3kDa (POLR2J) (AA 1-117) protein (Strep Tag)Crystallography grade POLR2J Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB
Polymerase (RNA) II (DNA Directed) Polypeptide J, 13.3kDa (POLR2J) protein (Myc-DYKDDDDK Tag)POLR2J Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Comment
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Synonyms: DNA-directed RNA polymerase II subunit RPB11-a, hRPB14, POLR2J1, RPB11, RPB11A, RPB11m
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/ml (determined by Bradford assay)
- Buffer
- 20 mM Tris-HCl buffer (pH 8.0) containing 0.2 M NaCl, 40% glycerol, 5 MM DTT, and 2 mM EDTA
- Storage
- 4 °C
- Storage Comment
- Avoid repeated freezing and thawing cycles.
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- Target
- POLR2J (Polymerase (RNA) II (DNA Directed) Polypeptide J, 13.3kDa (POLR2J))
- Alternative Name
- POLR2J (POLR2J Products)
- Synonyms
- POLR2J1 Protein, RPB11 Protein, RPB11A Protein, RPB11m Protein, hRPB14 Protein, POLR2J2 Protein, 14.5kDa Protein, Polr2i Protein, Rpb11a Protein, Rpo2-4 Protein, RNA polymerase II subunit J Protein, polymerase (RNA) II (DNA directed) polypeptide J, 13.3kDa S homeolog Protein, polymerase (RNA) II (DNA directed) polypeptide J, 13.3kDa Protein, polymerase (RNA) II (DNA directed) polypeptide J Protein, POLR2J Protein, Polr2j Protein, polr2j.S Protein, polr2j Protein
- Background
- POLR2J is a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This protein exists as a heterodimer with another polymerase subunit, together they form a core subassembly unit of the polymerase. Two similar genes are located nearby on chromosome 7q22.1 and a pseudogene is found on chromosome 7p13. Recombinant human POLR2J protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 15.7 kDa (140aa) confirmed by MALDI-TOF
- NCBI Accession
- NP_006225
- Pathways
- Regulatory RNA Pathways
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