POLR2E Protein (His tag)
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- Target See all POLR2E Proteins
- POLR2E (Polymerase (RNA) II (DNA Directed) Polypeptide E, 25kDa (POLR2E))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This POLR2E protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Purification
- purified by chromatography
- Purity
- > 90 % by SDS - PAGE
- Top Product
- Discover our top product POLR2E Protein
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- Comment
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Synonyms: DNA-directed RNA polymerases I, II, and III subunit RPABC1, hRPB25, hsRPB5, RPABC1, RPB5, XAP4
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/ml (determined by Bradford assay)
- Buffer
- 20 mM Tris-HCl buffer (pH 8.0) containing 0.1 M NaCl, 10% glycerol, 1 MM DTT
- Storage
- 4 °C
- Storage Comment
- Avoid repeated freezing and thawing cycles.
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- Target
- POLR2E (Polymerase (RNA) II (DNA Directed) Polypeptide E, 25kDa (POLR2E))
- Alternative Name
- POLR2E (POLR2E Products)
- Synonyms
- MGC68604 Protein, polr2e Protein, wu:fk97g10 Protein, zgc:101098 Protein, 2410021N14Rik Protein, 25kDa Protein, AW208866 Protein, RPB5 Protein, XAP4 Protein, RPABC1 Protein, hRPB25 Protein, hsRPB5 Protein, polymerase (RNA) II (DNA directed) polypeptide E, 25kDa S homeolog Protein, RNA polymerase II subunit E Protein, polymerase (RNA) II (DNA directed) polypeptide E, b Protein, polymerase (RNA) II (DNA directed) polypeptide E, 25kDa Protein, polymerase (RNA) II (DNA directed) polypeptide E Protein, DNA-directed RNA polymerases I, II, and III subunit RPABC1 Protein, polr2e.S Protein, POLR2E Protein, polr2eb Protein, polr2e Protein, Polr2e Protein, rpb-5 Protein
- Background
- POLR2E, also as known as DNA-directed RNA polymerases I, II, and III subunit RPABC1, belongs to the archaeal RpoH/eukaryotic RPB5 RNA polymerase subunit family. POLR2E is the fifth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit is shared by the other two DNA-directed RNA polymerases and is present in two-fold molar excess over the other polymerase subunits. POLR2E is a DNA-dependent RNA polymerase that catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Recombinant human POLR2E protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 27.1kDa (233aa), confirmed by MALDI-TOF
- NCBI Accession
- NP_002686
- Pathways
- Regulatory RNA Pathways
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