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POLR2E Protein (His tag)

POLR2E Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % by SDS - PAGE SDS
Catalog No. ABIN1098477
  • Target See all POLR2E Proteins
    POLR2E (Polymerase (RNA) II (DNA Directed) Polypeptide E, 25kDa (POLR2E))
    Protein Type
    Recombinant
    Origin
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Source
    • 6
    • 2
    • 1
    • 1
    Escherichia coli (E. coli)
    Purification tag / Conjugate
    This POLR2E protein is labelled with His tag.
    Application
    SDS-PAGE (SDS)
    Purification
    purified by chromatography
    Purity
    > 90 % by SDS - PAGE
    Top Product
    Discover our top product POLR2E Protein
  • Comment

    Synonyms: DNA-directed RNA polymerases I, II, and III subunit RPABC1, hRPB25, hsRPB5, RPABC1, RPB5, XAP4

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/ml (determined by Bradford assay)
    Buffer
    20 mM Tris-HCl buffer (pH 8.0) containing 0.1 M NaCl, 10% glycerol, 1 MM DTT
    Storage
    4 °C
    Storage Comment
    Avoid repeated freezing and thawing cycles.
  • Target
    POLR2E (Polymerase (RNA) II (DNA Directed) Polypeptide E, 25kDa (POLR2E))
    Alternative Name
    POLR2E (POLR2E Products)
    Synonyms
    MGC68604 Protein, polr2e Protein, wu:fk97g10 Protein, zgc:101098 Protein, 2410021N14Rik Protein, 25kDa Protein, AW208866 Protein, RPB5 Protein, XAP4 Protein, RPABC1 Protein, hRPB25 Protein, hsRPB5 Protein, polymerase (RNA) II (DNA directed) polypeptide E, 25kDa S homeolog Protein, RNA polymerase II subunit E Protein, polymerase (RNA) II (DNA directed) polypeptide E, b Protein, polymerase (RNA) II (DNA directed) polypeptide E, 25kDa Protein, polymerase (RNA) II (DNA directed) polypeptide E Protein, DNA-directed RNA polymerases I, II, and III subunit RPABC1 Protein, polr2e.S Protein, POLR2E Protein, polr2eb Protein, polr2e Protein, Polr2e Protein, rpb-5 Protein
    Background
    POLR2E, also as known as DNA-directed RNA polymerases I, II, and III subunit RPABC1, belongs to the archaeal RpoH/eukaryotic RPB5 RNA polymerase subunit family. POLR2E is the fifth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit is shared by the other two DNA-directed RNA polymerases and is present in two-fold molar excess over the other polymerase subunits. POLR2E is a DNA-dependent RNA polymerase that catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Recombinant human POLR2E protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
    Molecular Weight
    27.1kDa (233aa), confirmed by MALDI-TOF
    NCBI Accession
    NP_002686
    Pathways
    Regulatory RNA Pathways
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