C2orf60 Protein (His tag)

Catalog No. ABIN1098482

Product Details

Target: C2orf60 (Chromosome 2 Open Reading Frame 60 (C2orf60))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This C2orf60 protein is labelled with His tag.

Application: SDS-PAGE (SDS)

Purification: purified by chromatography

Purity: > 95 % by SDS - PAGE

Application Details

Comment:

Synonyms: tRNA wybutosine-synthesizing protein 5, C2orf60, Htyw5

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/ml (determined by Bradford assay)

Buffer: 20 mM Tris-HCl buffer (pH 8.0) containing 0.15 M NaCl, 10% glycerol, 1 MM DTT

Storage: 4 °C

Storage Comment: Avoid repeated freezing and thawing cycles.

Target Details

Target: C2orf60 (Chromosome 2 Open Reading Frame 60 (C2orf60))

Alternative Name: TYW5 (C2orf60 Products)

Synonyms: JMJD5 Protein, C2orf60 Protein, hTYW5 Protein, RGD1311269 Protein, C7H2orf60 Protein, 1110034B05Rik Protein, tRNA-yW synthesizing protein 5 Protein, TYW5 Protein, Tyw5 Protein

Background: TRNA wybutosine-synthesizing protein 5, also known as TYW5, acts as a component of the wybutosine biosynthesis pathway. Wybutosine is a hyper modified guanosine with a tricyclic base found at the 3'-position adjacent to the anticodon of eukaryotic phenylalanine tRNA. TYW5 catalyzes the hydroxylation of 7-(a-amino-a-carboxypropyl)wyosine (yW-72) into undermodified hydroxywybutosine (OHyW). OHyW is a derivative of wybutosine found in higher eukaryotes. Recombinant human TYW5 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Molecular Weight: 38.9kDa (338aa), confirmed by MALDI-TOF

NCBI Accession: NP_001034782

Pathways: Ribonucleoside Biosynthetic Process