MED20 Protein (His tag)
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- Target See all MED20 Proteins
- MED20 (Mediator Complex Subunit 20 (MED20))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This MED20 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Purification
- purified by chromatography
- Purity
- > 90 % by SDS - PAGE
- Top Product
- Discover our top product MED20 Protein
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Mediator Complex Subunit 20 (MED20) (AA 1-212) protein (His tag)
MED20 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Mediator Complex Subunit 20 (MED20) (AA 1-212) protein (GST tag)MED20 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Mediator Complex Subunit 20 (MED20) (AA 1-212) protein (Strep Tag)Crystallography grade MED20 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
Mediator Complex Subunit 20 (MED20) (AA 1-212) protein (Strep Tag)Crystallography grade MED20 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
Mediator Complex Subunit 20 (MED20) protein (Myc-DYKDDDDK Tag)MED20 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Comment
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Synonyms: Mediator of RNA polymerase II transcription subunit 20, PRO0213, TRFP
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.25 mg/ml (determined by Bradford assay)
- Buffer
- 20 mM Tris-HCl buffer (pH 8.5) containing 0.2 M NaCl, 50% glycerol, 1 MM DTT
- Storage
- 4 °C
- Storage Comment
- Avoid repeated freezing and thawing cycles.
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- Target
- MED20 (Mediator Complex Subunit 20 (MED20))
- Alternative Name
- MED20 (MED20 Products)
- Synonyms
- PRO0213 Protein, TRFP Protein, 1110011O05Rik Protein, 2410115I17Rik Protein, AU018348 Protein, Trfp Protein, Usp49 Protein, trfp Protein, zgc:101043 Protein, mediator complex subunit 20 Protein, mediator complex subunit 20 S homeolog Protein, MED20 Protein, Med20 Protein, med20 Protein, med20.S Protein
- Background
- MED20 is a subunit of the Mediator complex, a multiprotein coactivator of RNA transcription that interacts with DNA-bound transcriptional activators, RNA polymerase II (see MIM 180660), and general initiation factors. Recombinant human MED20 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 25 kDa (235aa), confirmed by MALDI-TOF
- NCBI Accession
- NP_004266
- Pathways
- Regulation of Lipid Metabolism by PPARalpha
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