POLR2J2 Protein (His tag)
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- Target See all POLR2J2 Proteins
- POLR2J2 (Polymerase (RNA) II (DNA Directed) Polypeptide J2 (POLR2J2))
- Protein Type
- Recombinant
- Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This POLR2J2 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Purification
- purified by chromatography
- Purity
- > 95 % by SDS - PAGE
- Top Product
- Discover our top product POLR2J2 Protein
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Polymerase (RNA) II (DNA Directed) Polypeptide J2 (POLR2J2) (AA 1-115) protein (His tag)
POLR2J2 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Polymerase (RNA) II (DNA Directed) Polypeptide J2 (POLR2J2) (AA 1-116) protein (GST tag)POLR2J2 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Polymerase (RNA) II (DNA Directed) Polypeptide J2 (POLR2J2) (AA 1-115) protein (GST tag)POLR2J2 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Polymerase (RNA) II (DNA Directed) Polypeptide J2 (POLR2J2) (AA 1-115) protein (Strep Tag)Crystallography grade POLR2J2 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, ELISA, SDS
Polymerase (RNA) II (DNA Directed) Polypeptide J2 (POLR2J2) protein (Myc-DYKDDDDK Tag)POLR2J2 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Comment
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Synonyms: DNA-directed RNA polymerase II subunit RPB11-b1, HRPB11B, RPB11b1
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.25 mg/ml (determined by Bradford assay)
- Buffer
- 20 mM Tris-HCl buffer (pH 8.0) containing 0.15 M NaCl, 10% glycerol, 1 MM DTT, 250 mM Imidazole
- Storage
- 4 °C
- Storage Comment
- Avoid repeated freezing and thawing cycles.
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- Target
- POLR2J2 (Polymerase (RNA) II (DNA Directed) Polypeptide J2 (POLR2J2))
- Alternative Name
- POLR2J2 (POLR2J2 Products)
- Synonyms
- HRPB11B Protein, RPB11b1 Protein, RNA polymerase II subunit J2 Protein, POLR2J2 Protein
- Background
- POLR2J2 is a member of the RNA polymerase II subunit 11 gene family, which includes three genes in a cluster on chromosome 7q22.1 and a pseudogene on chromosome 7p13. The founding member of family, DNA directed RNA polymerase II polypeptide J, has been shown to encode a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This locus produces multiple, alternatively spliced transcripts that potentially express isoforms with distinct C-termini compared to DNA directed RNA polymerase II polypeptide J. Most or all variants are spliced to include additional non-coding exons at the 3' end which makes them candidates for nonsense-mediated decay (NMD). Consequently, it is not known if this locus expresses a protein or proteins in vivo. Recombinant human POLR2J2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 15.5 kDa (138aa) confirmed by MALDI-TOF
- NCBI Accession
- NP_116581
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