Prohibitin Protein (PHB) (His tag)
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- Target See all Prohibitin (PHB) Proteins
- Prohibitin (PHB)
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This Prohibitin protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Purification
- purified by chromatography
- Purity
- > 85 % by SDS - PAGE
- Top Product
- Discover our top product PHB Protein
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Prohibitin (PHB) (AA 1-272) protein (GST tag)
PHB Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Prohibitin (PHB) (AA 1-272) protein (His-IF2DI Tag)PHB Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Prohibitin (PHB) (AA 2-272) protein (His tag)Crystallography grade PHB Origin: Human Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Prohibitin (PHB) (AA 2-272) protein (His tag)Crystallography grade PHB Origin: Mouse Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Prohibitin (PHB) (AA 1-272), (full length) protein (His tag)PHB Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
Prohibitin (PHB) protein (Myc-DYKDDDDK Tag)PHB Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Comment
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Synonyms: Prohibitin, PHB1
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/ml (determined by Bradford assay)
- Buffer
- 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol 0.1 M NaCl
- Storage
- 4 °C
- Storage Comment
- Avoid repeated freezing and thawing cycles.
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- Target
- Prohibitin (PHB)
- Alternative Name
- PHB (PHB Products)
- Synonyms
- phb1 Protein, MGC75944 Protein, PHB Protein, Phb Protein, ACYPI000080 Protein, phb Protein, PHB1 Protein, Bap32 Protein, sr:nyz128 Protein, wu:fa18f07 Protein, wu:fb09f05 Protein, prohibitin1 Protein, xphb1 Protein, prohibitin Protein, prohibitin-2 Protein, prohibitin L homeolog Protein, PHB Protein, phb Protein, LOC484431 Protein, LOC5576054 Protein, Phb Protein, phb.L Protein
- Background
- PHB is an evolutionarily conserved gene that is ubiquitously expressed. It is thought to be a negative regulator of cell proliferation and may be a tumor suppressor. Mutations in PHB have been linked to sporadic breast cancer. Prohibitin is expressed as two transcripts with varying lengths of 3' untranslated region. The longer transcript is present at higher levels in proliferating tissues and cells, suggesting that this longer 3' untranslated region may function as a trans-acting regulatory RNA. Recombinant human PHB protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 31.9 kDa(292aa) confirmed by MALDI-TOF
- NCBI Accession
- NP_002625
- Pathways
- Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling
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