UBE2M Protein (His tag)
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- Target See all UBE2M (ube2m) Proteins
- UBE2M (ube2m) (Ubiquitin-Conjugating Enzyme E2M (ube2m))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This UBE2M protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Purification
- purified by using conventional chromatography.
- Purity
- > 95 % by SDS - PAGE
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Ubiquitin-Conjugating Enzyme E2M (ube2m) (AA 1-183) protein (GST tag)
ube2m Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
Ubiquitin-Conjugating Enzyme E2M (ube2m) (AA 1-183) protein (Strep Tag)Crystallography grade ube2m Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB
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- Comment
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Synonyms: NEDD8-conjugating enzyme Ubc12, hUbc12, UBC-RS2, UBC12
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/ml (determined by Bradford assay)
- Buffer
- 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.2 M NaCl, 1 MM DTT
- Storage
- 4 °C
- Storage Comment
- Avoid repeated freezing and thawing cycles.
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- Target
- UBE2M (ube2m) (Ubiquitin-Conjugating Enzyme E2M (ube2m))
- Alternative Name
- UBE2M (ube2m Products)
- Synonyms
- DDBDRAFT_0204633 Protein, DDBDRAFT_0238042 Protein, DDB_0204633 Protein, DDB_0238042 Protein, UBC-RS2 Protein, UBC12 Protein, hUbc12 Protein, 2510040H03Rik Protein, Ubc-rs2 Protein, ubiquitin conjugating enzyme E2M Protein, ubiquitin-conjugating enzyme E2M Protein, ubiquitin conjugating enzyme E2 M Protein, NEDD8-conjugating enzyme Protein, ubiquitin conjugating enzyme E2M S homeolog Protein, ube2m Protein, LOC692934 Protein, UBE2M Protein, Ube2m Protein, ube2m.S Protein
- Background
- UBE2M, also known as NEDD8-conjugating enzyme Ubc12, belongs to the ubiquitin-conjugating enzyme family. The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein is linked with a ubiquitin-like protein, NEDD8, which can be conjugated to cellular proteins, such as Cdc53/culin. Recombinant human UBE2M protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
- Molecular Weight
- 23.5 kDa (207aa) confirmed by MALDI-TOF
- NCBI Accession
- NP_003960
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