TK1 Protein (His tag)
-
- Target See all TK1 Proteins
- TK1 (Thymidine Kinase 1, Soluble (TK1))
- Protein Type
- Recombinant
-
Origin
- Human
-
Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This TK1 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Purification
- purified by using conventional chromatography.
- Purity
- > 90 % by SDS - PAGE
- Top Product
- Discover our top product TK1 Protein
-
Thymidine Kinase 1, Soluble (TK1) (AA 2-242) protein (His tag)
TK1 Origin: Dog Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by Tris-Bis PAGE
Thymidine Kinase 1, Soluble (TK1) (AA 2-234) protein (His tag)TK1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses ELISA, WB
Thymidine Kinase 1, Soluble (TK1) (AA 1-234) protein (GST tag)TK1 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Thymidine Kinase 1, Soluble (TK1) (AA 157-234) protein (GST tag)TK1 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Thymidine Kinase 1, Soluble (TK1) (AA 2-234) protein (His tag)Crystallography grade TK1 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB, Crys
Thymidine Kinase 1, Soluble (TK1) (AA 2-233) protein (His tag)Crystallography grade TK1 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB, Crys
Thymidine Kinase 1, Soluble (TK1) (AA 1-376), (full length) protein (His tag)TK1 Origin: Herpes Simplex Virus (HSV) Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
-
- Comment
-
Synonyms: Thymidine kinase, cytosolic, TK2
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 1 mg/ml (determined by Bradford assay)
- Buffer
- 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1 M NaCl, 1 MM DTT
- Storage
- 4 °C
- Storage Comment
- Avoid repeated freezing and thawing cycles.
-
- Target
- TK1 (Thymidine Kinase 1, Soluble (TK1))
- Alternative Name
- TK1 (TK1 Products)
- Synonyms
- fi19b06 Protein, zgc:55362 Protein, wu:fi19b06 Protein, TK1 Protein, tk2 Protein, D530002A18Rik Protein, Tk-1 Protein, Tk1a Protein, Tk1b Protein, TK2 Protein, Tk Protein, TK Protein, thymidine kinase 1, soluble Protein, thymidine kinase 1 Protein, thymidine kinase, cytosolic Protein, Thymidine kinase, cytosolic Protein, temporal expression: early Protein, thymidine kinase Protein, Tk thymidine kinase Protein, tk1 Protein, TK1 Protein, EDI_049980 Protein, kith Protein, J2R Protein, HVT031 Protein, Tk1 Protein, tk Protein
- Background
- TK1, also known as thymidine kinase, is an enzyme, a phosphotransferase (a kinase): 2'-deoxythymidine kinase, ATP-thymidine 5'-phosphotransferase. It is present in two forms in mammalian cells, TK1 and TK2. Thymidine kinases have a key function in the synthesis of DNA and thereby in cell division, as they are part of the unique reaction chain to introduce deoxythymidine into the DNA. Deoxythymidine is present in the body fluids as a result of degradation of DNA from food and from dead cells. Thymidine kinase is required for the action of many antiviral drugs. It is used to select hybridoma cell lines in production of monoclonal antibodies. In clinical chemistry it is used as a proliferation marker in the diagnosis, control of treatment and follow-up of malignant disease, mainly of hematological malignancies. Recombinant human TK1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
- Molecular Weight
- 28.0 kDa (258aa) confirmed by MALDI-TOF
- NCBI Accession
- NP_003249
-
