LCAT Protein (His tag)
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- Target See all LCAT Proteins
- LCAT (Lecithin-Cholesterol Acyltransferase (LCAT))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This LCAT protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Purity
- > 85 % by SDS - PAGE
- Top Product
- Discover our top product LCAT Protein
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Lecithin-Cholesterol Acyltransferase (LCAT) (AA 25-440) protein (His tag)
LCAT Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Lecithin-Cholesterol Acyltransferase (LCAT) (AA 25-438) protein (His tag)LCAT Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Lecithin-Cholesterol Acyltransferase (LCAT) (AA 1-440) protein (GST tag)LCAT Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Lecithin-Cholesterol Acyltransferase (LCAT) (AA 98-197) protein (GST tag)LCAT Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Lecithin-Cholesterol Acyltransferase (LCAT) (AA 25-440) protein (His tag)Crystallography grade LCAT Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Lecithin-Cholesterol Acyltransferase (LCAT) (AA 25-438) protein (His tag)Crystallography grade LCAT Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
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- Comment
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Synonyms: Phosphatidylcholine-sterol acyltransferase, lecithin-cholesterol acyltransferase, phosphatidylcholine-sterol O-acyltransferase
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/ml (determined by Bradford assay)
- Buffer
- 20 mM Tris-HCl buffer (pH 8.0) containing 0.4 M Urea, 10% glycerol
- Storage
- 4 °C
- Storage Comment
- Avoid repeated freezing and thawing cycles.
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- Target
- LCAT (Lecithin-Cholesterol Acyltransferase (LCAT))
- Alternative Name
- LCAT (LCAT Products)
- Synonyms
- AI046659 Protein, D8Wsu61e Protein, MGC82035 Protein, lcat Protein, MGC88964 Protein, LCAT Protein, lecithin-cholesterol acyltransferase Protein, lecithin cholesterol acyltransferase Protein, lecithin-cholesterol acyltransferase L homeolog Protein, solute carrier family 12 member 4 Protein, fragile site, aphidicolin type, common, fra(13)(q13.2) Protein, LCAT Protein, Lcat Protein, lcat.L Protein, lcat Protein, SLC12A4 Protein, FRA13A Protein
- Background
- LCAT (lecithin-cholesterol acyltransferase), also known as phosphatidylcholine-sterol O-acyltransferase, belongs to the AB hydrolase superfamily. LCAT is responsible for the esterification of the free cholesterol of plasma lipoproteins. This enzyme is converts free cholesterol into cholesteryl ester (a more hydrophobic form of cholesterol), which is then sequestered into the core of a lipoprotein particle, eventually making the newly synthesized HDL spherical and forcing the reaction to become unidirectional since the particles are removed from the surface. It is central enzyme in the extracellular metabolism of plasma lipoproteins and required for remodeling high-density lipoprotein particles into their spherical forms. Recombinant human LCAT protein, fused to His-tag at N-terminus, was expressed in E.coli.
- Molecular Weight
- 49.8kDa (441aa)
- NCBI Accession
- NP_000220
- Pathways
- Lipid Metabolism
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