CPSF4 Protein (His tag)
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- Target See all CPSF4 Proteins
- CPSF4 (Cleavage and Polyadenylation Specific Factor 4, 30kDa (CPSF4))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This CPSF4 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Purity
- > 85 % by SDS - PAGE
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Cleavage and Polyadenylation Specific Factor 4, 30kDa (CPSF4) (AA 1-244) protein (GST tag)
CPSF4 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
Cleavage and Polyadenylation Specific Factor 4, 30kDa (CPSF4) (AA 1-269) protein (Strep Tag)Crystallography grade CPSF4 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB
Cleavage and Polyadenylation Specific Factor 4, 30kDa (CPSF4) (AA 1-211) protein (Strep Tag)Crystallography grade CPSF4 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB
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- Comment
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Synonyms: cleavage and polyadenylation specific factor 4, 30kDa, CPSF30, NAR, NEB1
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/ml (determined by Bradford assay)
- Buffer
- 20 mM Tris-HCl buffer (pH 8.0) containing 0.4 M UREA, 10% glycerol
- Storage
- 4 °C
- Storage Comment
- Avoid repeated freezing and thawing cycles.
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- Target
- CPSF4 (Cleavage and Polyadenylation Specific Factor 4, 30kDa (CPSF4))
- Alternative Name
- CPSF4 (CPSF4 Products)
- Synonyms
- cpsf30 Protein, nar Protein, neb1 Protein, 30kDa Protein, C79664 Protein, CPSF30 Protein, NAR Protein, NEB1 Protein, cleavage and polyadenylation specific factor 4 Protein, cleavage and polyadenylation specific factor 4, 30kDa Protein, cleavage and polyadenylation specific factor 4 S homeolog Protein, CPSF4 Protein, Cpsf4 Protein, LOC733099 Protein, LOC100360200 Protein, cpsf4.S Protein, cpsf4 Protein
- Background
- Inhibition of the nuclear export of poly(A)-containing mRNAs caused by the influenza A virus NS1 protein requires its effector domain. The NS1 effector domain functionally interacts with the cellular 30 kDa subunit of CPSF4 an essential component of the 3' end processing machinery of cellular pre-mRNAs. In influenza virus-infected cells, the NS1 protein is physically associated with cleavage and polyadenylation specific factor 4, 30kD subunit. Binding of the NS1 protein to the 30 kDa protein in vitro prevents CPSF binding to the RNA substrate and inhibits 3' end cleavage and polyadenylation of host pre-mRNAs. Recombinant human CPSF4 protein, fused to His-tag at N-terminus, was expressed in E.coli.
- Molecular Weight
- 29.9 kDa (267aa)
- NCBI Accession
- NP_001075028
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