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RANKL Protein (AA 70 – 244) (His tag)

TNFSF11 Origin: Human Recombinant > 97 % by SDS-PAGE gel WB, Imm, CC
Catalog No. ABIN1112026
  • Target See all RANKL (TNFSF11) Proteins
    RANKL (TNFSF11) (Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 70 – 244
    Origin
    • 33
    • 22
    • 4
    • 2
    Human
    Host
    Please inquire
    Purification tag / Conjugate
    This RANKL protein is labelled with His tag.
    Application
    Western Blotting (WB), Immunogen (Imm), Cell Culture (CC)
    Sequence
    HHHHHHHHHH EKAMVDGSWL DLAKRSKLEA QPFAHLTINA TDIPSGSHKV SLSSWYHDRG WAKISNMTFS NGKLIVNQDG FYYLYANICF RHHETSGDLA TEYLQLMVYV TKTSIKIPSS HTLMKGGSTK YWSGNSEFHF YSINVGGFFK LRSGEEISIE VSNPSLLDPD QDATYFGAFK VRDID
    Specificity
    Serological Identification: The protein was electrophoresed under reducing condition on a 15 % SDS-polyacrylamide gel, transferred by electro blotting to a NC membrane and visualized by immune-detection with specific RANKL antibody.
    Characteristics
    Human recombinant protein expressed in Nicotiana benthamiana. Recombinant human Receptor activator of nuclear factor kappa-B ligand (sRANK-ligand) contains a 10-His tag at the N-terminal end, is produced by transient expression in non-transgenic plants. This product contains no animal-derived components or impurities. Animal free product.
    Ext. Coeff. Abs (280nm) 0.1 % (=1g/l): E 0.1 % (1g/L) = 1.678 (A 280 nm)
    p.I.: 6,82
    Biological Activity: The specific activity is determined by the dose-dependent stimulation of IL-8 production in human PBMC. Activity results may vary with PBMC donors.
    Biological Activity ng/ml: Human PBMC stimulated with 10 ng/ml of Human recombinant sRANKL increases in three times the basal production of IL-8.
    Purification
    sequential chromatography (FPLC)
    Purity
    > 97 % by SDS-PAGE gel
    Endotoxin Level
    < 0.04 EU/µg protein (LAL method)
    Top Product
    Discover our top product TNFSF11 Protein
  • Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Lyophilized protein should be reconstituted in water following instructions of batch Quality Control sheet. At higher concentrations the solubility may be reduced and multimers generated. Optimal concentration should be determined for specific application and cell lines.
    Buffer
    10 mM Phosphate Potasium buffer pH 8 and 0.2 M NaCl
    Handling Advice
    Reconstituted protein should be stored in working aliquots at -20 °C. Repeated freezing and thawing is not recommended.
    Storage
    4 °C
  • DAmelio, Isaia, Isaia: "The osteoprotegerin/RANK/RANKL system: a bone key to vascular disease." in: Journal of endocrinological investigation, Vol. 32, Issue 4 Suppl, pp. 6-9, (2009) (PubMed).

    Takahashi, Udagawa, Suda: "A new member of tumor necrosis factor ligand family, ODF/OPGL/TRANCE/RANKL, regulates osteoclast differentiation and function." in: Biochemical and biophysical research communications, Vol. 256, Issue 3, pp. 449-55, (1999) (PubMed).

    Suda, Takahashi, Udagawa, Jimi, Gillespie, Martin: "Modulation of osteoclast differentiation and function by the new members of the tumor necrosis factor receptor and ligand families." in: Endocrine reviews, Vol. 20, Issue 3, pp. 345-57, (1999) (PubMed).

    Yasuda, Shima, Nakagawa, Yamaguchi, Kinosaki, Mochizuki, Tomoyasu, Yano, Goto, Murakami, Tsuda, Morinaga, Higashio, Udagawa, Takahashi, Suda: "Osteoclast differentiation factor is a ligand for osteoprotegerin/osteoclastogenesis-inhibitory factor and is identical to TRANCE/RANKL." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 7, pp. 3597-602, (1998) (PubMed).

  • Target
    RANKL (TNFSF11) (Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11))
    Abstract
    TNFSF11 Products
    Synonyms
    CD254 Protein, ODF Protein, OPGL Protein, OPTB2 Protein, RANKL Protein, TRANCE Protein, hRANKL2 Protein, sOdf Protein, TNFSF11 Protein, Ly109l Protein, OPG Protein, Trance Protein, cd254 Protein, odf Protein, opgl Protein, optb2 Protein, rankl Protein, sodf Protein, tnfsf11 Protein, trance Protein, TNF superfamily member 11 Protein, tumor necrosis factor (ligand) superfamily, member 11 Protein, TNF receptor superfamily member 11b Protein, TNF superfamily member 11 L homeolog Protein, TNFSF11 Protein, Tnfsf11 Protein, TNFRSF11B Protein, tnfsf11.L Protein
    Background
    Synonyms: Tumor necrosis factor ligand superfamily member 11 (TNFSF11), Osteoprotegerin ligand (OPGL), TNF-related activation-induced cytokine (TRANCE)
    Recombinant human RANKL is a member of TNF super family, a cytokine that play a central role in bone remodelling and disorders of mineral metabolism. It was shown to be a dendritic cell survival factor, T-cell activator and osteoclast regulator because RANKL mediates the osteoclast differentiation, survival and activation. Native RANKL is a type II trans-membrane protein with an extracellular binding domain that interacts with RANK and OPG receptors. OPG protects the skeleton from excessive bone resorption by binding to RANKL and preventing it from binding to its receptor, RANK. Thus, RANKL/OPG ratio became an important determinant of bone mass and skeletal integrity. In addition, this protein was shown to activate anti-apoptotic kinase AKT/PKB through a signalling complex involving SRC kinase and tumor necrosis factor receptor-associated factor (TREAF). Recent findings shown that OPG/RANK/RANKL system has been identifies as a possible mediator of arterial calcification suggesting common links between osteoporosis and vascular diseases.
    Molecular Weight
    21.1 kDa
    Pathways
    NF-kappaB Signaling
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