LTA Protein

Catalog No. ABIN1305059

Product Details

Target: LTA (Lymphotoxin-alpha (LTA))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Application: ELISA, Functional Studies (Func)

Brand: BD Pharmingen™

Specificity: Biological Activity: Bioactivity is tested in MTT/actinomycin D cytolysis assay using L929 indicator cells.
Specific Activity Range: 0.3 - 5.0x10^9 Unit /mg
ED50: 2 - 30 pg/ml
Observed dose response: > 500 fold
Unit is defined as the amount of material required to stimulate a half-maximal response at cytokine saturation.

Characteristics: Recombinant human LT-alpha is supplied as a frozen liquid comprised of 0.22 µm sterile-filtered Dulbecco’s PBS (pH 7.2) and containing biotechnology grade, low endotoxin bovine serum albumin, with no preservatives. The recombinant human LT-alpha is >95% pure as determined by SDS-PAGE analysis, and an absorbance assay based on the Beers-Lambert Law.

Purification: Recombinant human LT-α is supplied as a frozen liquid comprised of 0.22 μm sterile-filtered aqueous buffered solution, containing bovine serum albumin, with no preservatives. Recombinant human LT-α is ≥ 95 % pure as determined by SDS-PAGE analysis, and an absorbance assay based on the Beers-Lambert Law.

Purity: ≥ 95 %

Sterility: 0.22 μm filtered

Endotoxin Level: The endotoxin level is ≤ 0.1 ng per µg of LT-alpha, as measured in a chromogenic LAL assay.

Application Details

Application Notes: ELISA Standard: This recombinant human LT-alpha is useful as a quantitative standard for measuring human LT-alpha protein levels in a LT-alpha specific sandwich ELISA with the purified 359-238-8 antibody (Cat. No. 551222) as a capture antibody and the biotinylated 359-81-11 antibody (Cat. No. 554555) as the detection antibody.

Comment:

BD Pharmingen™ Recombinant Human LT-α - Reactivity Hu

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 200 μg/mL

Buffer: Frozen aqueous buffered solution containing BSA.

Handling Advice: Lymphotoxin (LT-α), also known as, Tumor Necrosis Factor-β (TNF-β) is a potent multifunctional cytokine which is primarily produced by activated lymphocytes. LT-α has cytotoxic activity against a wide range of tumor cells and certain other target cells. Other LT-α activities include the activation of neutrophils, osteoclasts, and endothelial cells and the upregulation of the membrane expression of MHC antigens and adhesion molecules by various cell types. Human LT-α is a 18 kDa protein containing 171 amino acid residues.

Storage: -80 °C

Storage Comment: Store product at -80°C prior to use or for long term storage of stock solutions. Rapidly thaw and quick-spin product prior to use. Avoid multiple freeze-thaws of product. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

References

Paul, Ruddle: "Lymphotoxin." in: Annual review of immunology, Vol. 6, pp. 407-38, (1988) (PubMed).

Target Details

Target: LTA (Lymphotoxin-alpha (LTA))

Alternative Name: LT-alpha (LTA Products)

Synonyms: LT Protein, TNFB Protein, TNFSF1 Protein, LT-[a] Protein, LT-alpha Protein, LT[a] Protein, LTalpha Protein, Ltx Protein, TNF-beta Protein, Tnfb Protein, Tnfsf1b Protein, hlb382 Protein, lymphotoxin alpha Protein, lymphotoxin A Protein, LTA Protein, Lta Protein

Target Type: Chemical

Background: Lymphotoxin (LT-α), also known as, Tumor Necrosis Factor-β (TNF-β) is a potent multifunctional cytokine which is primarily produced by activated lymphocytes. LT-α has cytotoxic activity against a wide range of tumor cells and certain other target cells. Other LT-α activities include the activation of neutrophils, osteoclasts, and endothelial cells and the upregulation of the membrane expression of MHC antigens and adhesion molecules by various cell types. Human LT-α is a 18 kDa protein containing 171 amino acid residues.

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process