IL12 Protein

Catalog No. ABIN1305066

Product Details

Target: IL12 (Interleukin 12 (IL12))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: Escherichia coli (E. coli)

Application: ELISA, Functional Studies (Func)

Brand: BD Pharmingen™

Specificity: Biological Activity Range: Measured by 2D6 cell proliferation Specific Activity: 0.2 - 2.0 x 10^9 Units /mg
ED50: 5 - 50 pg/ml
Observed dose response: > 200 fold
Unit is defined as the amount of material required to stimulate a half-maximal response at cytokine saturation

Characteristics: The recombinant mouse IL-12 was lyophilized in a solution comprised of 0.22 µm sterile-filtered aqueous buffered solution containing 2.0 mg/ml Biotechnology grade, low endotoxin bovine serum albumin, and containing no preservatives. The mass ratio of carrier-protein to mouse IL-12 is 40:1 or 40 µg BSA to 1 µg IL-12. Recombinant mouse IL-12 was found to be > 95% pure by SDS-PAGE and an absorbance assay based on the Beer-Lambert law.

Purification: Recombinant mouse IL-12 p70 is provided lyophilized, containing bovine serum albumin and no preservatives. Recombinant mouse IL-12 p70 was found to be ≥ 95 % pure as determined by SDS-PAGE, and an absorbance assay based on the Beer-Lambert law.

Purity: ≥ 95 %

Sterility: 0.22 μm filtered

Endotoxin Level: The endotoxin level is ≤ 0.1 ng per µg of mouse IL-12, as measured in a chromogenic LAL assay.

Application Details

Application Notes: ELISA Standard: This mouse IL-12 is useful as a quantitative standard for measuring mouse IL-12 protein levels in an IL-12 specific sandwich ELISA with the purified 9A5 antibody (Cat. No. 554658) as a capture antibody and the biotinylated C17.8 (Cat. No. 554476) as the detection antibody. To obtain linear standard curves, doubling dilutions of this mouse IL-12 standard from 4000-30 pg/ml should be included in each ELISA plate.

Comment:

BD Pharmingen™ Recombinant Mouse IL-12 p70 - Purified - Clone NA - Reactivity Ms

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized in an aqueous buffered solution containing BSA.

Handling Advice: Interleukin-12 (IL-12) is a potent regulator of cell-mediated immune responses. Biologically active IL-12 is secreted by activated B lymphocytes and macrophages as a 70 kD heterodimeric glycoprotein comprised of disulfide-bonded 35 kD (p35) and 40 kD (p40) subunits. IL-12 binds to specific high affinity receptors and can, i) stimulate the growth of activated CD4+ and CD8+ T cells and natural killer (NK) cells, ii) promote the development of proinflammatory/Th1-like CD4+ cells and cytotoxic CD8+ T cells, iii) increase the lytic activity of NK cells, lymphokine-activated killer cells and antibody dependent cell-mediated cytotoxic cells, and, iv) induce NK and T cells to secrete large amounts of the proinflammatory cytokine, IFN-γ.

Storage: -80 °C

Storage Comment: Store product at -80°C prior to use or for long term storage of stock solutions. Avoid multiple freeze-thaws of product. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

References

Schoenhaut, Chua, Wolitzky, Quinn, Dwyer, McComas, Familletti, Gately, Gubler: "Cloning and expression of murine IL-12." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 148, Issue 11, pp. 3433-40, (1992) (PubMed).

Target Details

Target: IL12 (Interleukin 12 (IL12))

Alternative Name: IL-12 (p70) (IL12 Products)

Synonyms: CLMF Protein, CLMF2 Protein, IL-12B Protein, NKSF Protein, NKSF2 Protein, p40 Protein, Il-12b Protein, Il12p40 Protein, Il-12p40 Protein, Il12 Protein, IL-12p40 Protein, IL-12 Protein, LOC100217488 Protein, IL-12p35 Protein, Il-12a Protein, Ll12a Protein, p35 Protein, IL12p40 Protein, IL12B Protein, IL-12 p35 Protein, interleukin 12B Protein, interleukin 12A Protein, interleukin 12b Protein, interleukin 12a Protein, Interleukin 12 level Protein, IL12B Protein, IL12A Protein, Il12b Protein, Il12a Protein, IL12 Protein, il12a Protein

Background: Interleukin-12 (IL-12) is a potent regulator of cell-mediated immune responses. Biologically active IL-12 is secreted by activated B lymphocytes and macrophages as a 70 kD heterodimeric glycoprotein comprised of disulfide-bonded 35 kD (p35) and 40 kD (p40) subunits. IL-12 binds to specific high affinity receptors and can, i) stimulate the growth of activated CD4+ and CD8+ T cells and natural killer (NK) cells, ii) promote the development of proinflammatory/Th1-like CD4+ cells and cytotoxic CD8+ T cells, iii) increase the lytic activity of NK cells, lymphokine-activated killer cells and antibody dependent cell-mediated cytotoxic cells, and, iv) induce NK and T cells to secrete large amounts of the proinflammatory cytokine, IFN-γ.

Pathways: JAK-STAT Signaling, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome