Interferon gamma Protein (IFNG)

Catalog No. ABIN1305090

Product Details

Target: Interferon gamma (IFNG)

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: Escherichia coli (E. coli)

Application: ELISA, Functional Studies (Func)

Brand: BD Pharmingen™

Specificity: Bioassay: Activity Range is measured in an anti-viral assay, using L929 indicator cells and an MTT assay as read-out.
Specific Activity: 0.3 - 3.3 x 10^8 Units /mg
ED50: 30 - 300 pg/ml
Observed dose response: 20 fold
Unit is defined to be the amount of material required to stimulate a half-maximal response at cytokine saturation.

Characteristics: Recombinant mouse IFN-gamma is supplied as a frozen aqueous buffered solution containing 2 mg/ml biotechnology grade, glycerol, low endotoxin BSA, with no preservative. The IFN-gamma -containing culture supernatant was purified by immunoaffinity chromatography and yielded an IFN-gamma protein fraction which was found to be > 95% pure by SDS-PAGE, and an absorbance assay based on the Beers-Lambert law.

Purification: Immunoaffinity Chromatography
Recombinant mouse IFN-γ is supplied as a frozen liquid comprised of 0.22 μm sterile-filtered aqueous buffered solution, glycerol and bovine serum albumin, with no preservatives. Recombinant mouse IFN-γ is ≥ 95 % pure as determined by SDS-PAGE, and an absorbance assay based on the Beers-Lambert law.

Purity: ≥ 95 %

Sterility: 0.22 μm filtered

Endotoxin Level: The endotoxin level is < 0.1 ng per µg of IFN-gamma, as measured in a chromogenic LAL assay.

Application Details

Application Notes: ELISA Standard: Recombinant mouse IFN-gamma is useful as a quantitative standard for measuring mouse IFN-gamma protein levels in IFN-gamma specific sandwich ELISA with the purified R4-6A2 antibody (Cat. No. 551216) as a capture antibody and the biotinylated XMG1.2 antibody (Cat. No. 554410) as the detection antibody. To obtain linear standard curves, doubling dilutions of this mouse IFN-gamma standard from ~4,000 to 30 pg/ml should be included in each ELISA plate. For measuring mouse IFN-gamma in serum or plasma, we recommend our BD OptEIA™ Mouse IFN-gamma ELISA Set (Cat. No. 551866), or Kit (Cat. No. 558258) which have been optimized for this purpose.

Comment:

BD Pharmingen™ Recombinant Mouse IFN-γ - Reactivity Ms

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 200 μg/mL

Buffer: Frozen aqueous buffered solution containing BSA and glycerol.

Preservative: Azide free

Handling Advice: Interferon-γ (IFN-γ) is a potent multifunctional cytokine which is secreted by activated NK cells and CD4+TCRαβ+, CD8+TCRαβ+, and TCRγδ+ T cells and exerts its biological effects through specific binding to a single class of high affinity receptors. In addition to its antiviral effects, IFN-γ can upregulate a number of lymphoid cell functions including the antimicrobial and antitumor responses of macrophages, NK cells, and neutrophils. IFN-γ can exert strong regulatory influences on the proliferation, differentiation, and effector responses of B cell and T cell subsets. These influences can involve the capacity of IFN-γ to boost MHC class I and II expression by antigen-presenting cells as well as direct effects on B cells and T cells themselves. Under reduced SDS-PAGE conditions, recombinant mouse IFN-γ is a differentially glycosylated protein with a molecular mass of approximately 17 kD.

Storage: -80 °C

Storage Comment: Store product at -80°C prior to use or for long term storage of stock solutions. Rapidly thaw and quick-spin product prior to use. Avoid multiple freeze-thaws of product. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

References

Farrar, Schreiber: "The molecular cell biology of interferon-gamma and its receptor." in: Annual review of immunology, Vol. 11, pp. 571-611, (1993) (PubMed).

Gray, Goeddel: "Cloning and expression of murine immune interferon cDNA." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 80, Issue 19, pp. 5842-6, (1983) (PubMed).

Target Details

Target: Interferon gamma (IFNG)

Alternative Name: IFN-gamma (IFNG Products)

Synonyms: IFG Protein, IFI Protein, IFN-g Protein, Ifg Protein, IFNG2 Protein, IFN-gamma Protein, IFN-G Protein, IFNG Protein, IFNgamma Protein, TCRalpha Protein, INF-G Protein, ifng Protein, interferon gamma Protein, interferon, gamma 1-2 Protein, IFNG Protein, Ifng Protein, ifng1-2 Protein

Background: Interferon-γ (IFN-γ) is a potent multifunctional cytokine which is secreted by activated NK cells and CD4+TCRαβ+, CD8+TCRαβ+, and TCRγδ+ T cells and exerts its biological effects through specific binding to a single class of high affinity receptors. In addition to its antiviral effects, IFN-γ can upregulate a number of lymphoid cell functions including the antimicrobial and antitumor responses of macrophages, NK cells, and neutrophils. IFN-γ can exert strong regulatory influences on the proliferation, differentiation, and effector responses of B cell and T cell subsets. These influences can involve the capacity of IFN-γ to boost MHC class I and II expression by antigen-presenting cells as well as direct effects on B cells and T cells themselves. Under reduced SDS-PAGE conditions, recombinant mouse IFN-γ is a differentially glycosylated protein with a molecular mass of approximately 17 kD.

Pathways: Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy