Interferon gamma Protein (IFNG)
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- Target See all Interferon gamma (IFNG) Proteins
- Interferon gamma (IFNG)
- Protein Type
- Recombinant
- Biological Activity
- Active
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Origin
- Mouse
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Source
- Escherichia coli (E. coli)
- Application
- ELISA, Functional Studies (Func)
- Brand
- BD Pharmingen™
- Specificity
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Bioassay: Activity Range is measured in an anti-viral assay, using L929 indicator cells and an MTT assay as read-out.
Specific Activity: 0.3 - 3.3 x 10^8 Units /mg
ED50: 30 - 300 pg/ml
Observed dose response: 20 fold
Unit is defined to be the amount of material required to stimulate a half-maximal response at cytokine saturation. - Characteristics
- Recombinant mouse IFN-gamma is supplied as a frozen aqueous buffered solution containing 2 mg/ml biotechnology grade, glycerol, low endotoxin BSA, with no preservative. The IFN-gamma -containing culture supernatant was purified by immunoaffinity chromatography and yielded an IFN-gamma protein fraction which was found to be > 95% pure by SDS-PAGE, and an absorbance assay based on the Beers-Lambert law.
- Purification
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Immunoaffinity Chromatography
Recombinant mouse IFN-γ is supplied as a frozen liquid comprised of 0.22 μm sterile-filtered aqueous buffered solution, glycerol and bovine serum albumin, with no preservatives. Recombinant mouse IFN-γ is ≥ 95 % pure as determined by SDS-PAGE, and an absorbance assay based on the Beers-Lambert law. - Purity
- ≥ 95 %
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- The endotoxin level is < 0.1 ng per µg of IFN-gamma, as measured in a chromogenic LAL assay.
- Top Product
- Discover our top product IFNG Protein
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- Application Notes
- ELISA Standard: Recombinant mouse IFN-gamma is useful as a quantitative standard for measuring mouse IFN-gamma protein levels in IFN-gamma specific sandwich ELISA with the purified R4-6A2 antibody (Cat. No. 551216) as a capture antibody and the biotinylated XMG1.2 antibody (Cat. No. 554410) as the detection antibody. To obtain linear standard curves, doubling dilutions of this mouse IFN-gamma standard from ~4,000 to 30 pg/ml should be included in each ELISA plate. For measuring mouse IFN-gamma in serum or plasma, we recommend our BD OptEIA™ Mouse IFN-gamma ELISA Set (Cat. No. 551866), or Kit (Cat. No. 558258) which have been optimized for this purpose.
- Comment
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BD Pharmingen™ Recombinant Mouse IFN-γ - Reactivity Ms
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 200 μg/mL
- Buffer
- Frozen aqueous buffered solution containing BSA and glycerol.
- Preservative
- Azide free
- Handling Advice
- Interferon-γ (IFN-γ) is a potent multifunctional cytokine which is secreted by activated NK cells and CD4+TCRαβ+, CD8+TCRαβ+, and TCRγδ+ T cells and exerts its biological effects through specific binding to a single class of high affinity receptors. In addition to its antiviral effects, IFN-γ can upregulate a number of lymphoid cell functions including the antimicrobial and antitumor responses of macrophages, NK cells, and neutrophils. IFN-γ can exert strong regulatory influences on the proliferation, differentiation, and effector responses of B cell and T cell subsets. These influences can involve the capacity of IFN-γ to boost MHC class I and II expression by antigen-presenting cells as well as direct effects on B cells and T cells themselves. Under reduced SDS-PAGE conditions, recombinant mouse IFN-γ is a differentially glycosylated protein with a molecular mass of approximately 17 kD.
- Storage
- -80 °C
- Storage Comment
- Store product at -80°C prior to use or for long term storage of stock solutions. Rapidly thaw and quick-spin product prior to use. Avoid multiple freeze-thaws of product. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
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The molecular cell biology of interferon-gamma and its receptor." in: Annual review of immunology, Vol. 11, pp. 571-611, (1993) (PubMed).
: "Cloning and expression of murine immune interferon cDNA." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 80, Issue 19, pp. 5842-6, (1983) (PubMed).
: "
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The molecular cell biology of interferon-gamma and its receptor." in: Annual review of immunology, Vol. 11, pp. 571-611, (1993) (PubMed).
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- Target
- Interferon gamma (IFNG)
- Alternative Name
- IFN-gamma (IFNG Products)
- Synonyms
- IFG Protein, IFI Protein, IFN-g Protein, Ifg Protein, IFNG2 Protein, IFN-gamma Protein, IFN-G Protein, IFNG Protein, IFNgamma Protein, TCRalpha Protein, INF-G Protein, ifng Protein, interferon gamma Protein, interferon, gamma 1-2 Protein, IFNG Protein, Ifng Protein, ifng1-2 Protein
- Background
- Interferon-γ (IFN-γ) is a potent multifunctional cytokine which is secreted by activated NK cells and CD4+TCRαβ+, CD8+TCRαβ+, and TCRγδ+ T cells and exerts its biological effects through specific binding to a single class of high affinity receptors. In addition to its antiviral effects, IFN-γ can upregulate a number of lymphoid cell functions including the antimicrobial and antitumor responses of macrophages, NK cells, and neutrophils. IFN-γ can exert strong regulatory influences on the proliferation, differentiation, and effector responses of B cell and T cell subsets. These influences can involve the capacity of IFN-γ to boost MHC class I and II expression by antigen-presenting cells as well as direct effects on B cells and T cells themselves. Under reduced SDS-PAGE conditions, recombinant mouse IFN-γ is a differentially glycosylated protein with a molecular mass of approximately 17 kD.
- Pathways
- Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy
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