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CCL2 Protein

CCL2 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant ≥ 95 % ELISA, Func
Catalog No. ABIN1305100
  • Target See all CCL2 Proteins
    CCL2 (Chemokine (C-C Motif) Ligand 2 (CCL2))
    Protein Type
    Recombinant
    Origin
    • 23
    • 11
    • 8
    • 4
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Mouse
    Source
    • 34
    • 12
    • 10
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    Escherichia coli (E. coli)
    Application
    ELISA, Functional Studies (Func)
    Brand
    BD Pharmingen™
    Specificity
    Upon initial thawing, recombinant mouse MCP-1 (MN 554590) should be aliquoted into polypropylene microtubes and frozen at -80°C for future use. Alternatively, the product can be diluted in sterile neutral buffer containing not less than 0.5 - 10 mg/mL carrier protein, such as human or bovine serum albumin, aliquoted and stored at -80°C. For in vitro biological assay use, carrier-protein concentrations of 0.5 - 1 mg/mL are recommended. For use as an ELISA standard, carrier-protein concentrations of 5 - 10 mg/mL are recommended. Failure to add carrier protein or store at indicated temperatures may result in a loss of activity. Carrier proteins should be pre-screened for possible affects in each investigator's experimental system. Carrier proteins may have an undesired influence on experimental results due to toxicity, high endotoxin levels or possible blocking activity.
    Characteristics
    Recombinant mouse MCP-1 (MN 554590) is supplied as a frozen liquid comprised of 0.22 µm sterile-filtered aqueous buffered solution (pH 7.2) and containing 1.29 mg/mL bovine serum albumin, with no preservatives. Recombinant mouse MCP-1 is > 95% pure as determined by SDS-PAGE, and an absorbance assay based on the Beers-Lambert law.
    Purification
    Recombinant mouse MCP-1 is supplied as a frozen liquid comprised of 0.22 μm sterile-filtered aqueous buffered solution ( pH 7.2) and containing 1.29 mg/mL bovine serum albumin, with no preservatives. Recombinant mouse MCP-1 is > 95 % pure as determined by SDS-PAGE, and an absorbance assay based on the Beers-Lambert law.
    Purity
    ≥ 95 %
    Sterility
    0.22 μm filtered
    Endotoxin Level
    The endotoxin level is ≤ 0.1 ng per µg of mouse MCP-1, as measured in a chromogenic LAL assay.
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    Discover our top product CCL2 Protein
  • Application Notes
    ELISA Standard: Recombinant mouse MCP-1 (MN 554590) can be useful as a quantitative standard for measuring mouse MCP-1 protein levels using sandwich ELISA with the purified 2H5 antibody (Cat. No. 551217) as a capture antibody and the biotinylated 4E2/MCP antibody (Cat. No. 554444) as the detection antibody. To obtain linear standard curves, investigators may want to consider using doubling dilutions of recombinant mouse MCP-1 from 4000-30 pg/mL to be included for each ELISA plate. For measuring mouse MCP-1 in serum or plasma, investigators are highly encouraged to use the BD OptEIA™ Mouse MCP-1 Set (Cat. No. 555260).
    Bioassay: Investigators are advised that the Bioassay application is not routinely tested for this material and are highly encouraged to both titrate this material and include appropriate controls in relevant experiments. An activity range encompassing an ED50 = 50 - 800 ng/mL has previously been reported using THP-1 as indicator cells utilizing a calcium flux assay.
    Comment

    BD Pharmingen™ Recombinant Mouse MCP-1 - Reactivity Ms

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    100 μg/mL
    Buffer
    Frozen aqueous buffered solution containing BSA.
    Preservative
    Azide free
    Handling Advice
    Mouse monocyte chemoattractant protein-1 (MCP-1), previously termed JE, is a member of the CC chemokine family and a homolog of human MCP-1. MCP-1 is expressed by a variety of cell types including monocyte/macrophages, endothelial cells and mesangial cells. MCP-1 has chemoattractant activity on monocytes, lymphocytes and basophils but is not active on neutrophils.
    Storage
    -80 °C
    Storage Comment
    Store product at -80°C prior to use or for long term storage of stock solutions. Rapidly thaw and quick-spin product prior to use. Avoid multiple freeze-thaws of product. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
  • Rollins: "Chemokines." in: Blood, Vol. 90, Issue 3, pp. 909-28, (1997) (PubMed).

    Ernst, Zhang, Hancock, Rutledge, Corless, Rollins: "Biochemical and biologic characterization of murine monocyte chemoattractant protein-1. Identification of two functional domains." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 152, Issue 7, pp. 3541-9, (1994) (PubMed).

    Luo, Laning, Hayashi, Hancock, Rollins, Dorf: "Serologic analysis of the mouse beta chemokine JE/monocyte chemoattractant protein-1." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 153, Issue 8, pp. 3708-16, (1994) (PubMed).

    Rollins, Morrison, Stiles: "Cloning and expression of JE, a gene inducible by platelet-derived growth factor and whose product has cytokine-like properties." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 85, Issue 11, pp. 3738-42, (1988) (PubMed).

    Cochran, Reffel, Stiles: "Molecular cloning of gene sequences regulated by platelet-derived growth factor." in: Cell, Vol. 33, Issue 3, pp. 939-47, (1983) (PubMed).

  • Target
    CCL2 (Chemokine (C-C Motif) Ligand 2 (CCL2))
    Alternative Name
    MCP-1 (CCL2 Products)
    Synonyms
    GDCF-2 Protein, HC11 Protein, HSMCR30 Protein, MCAF Protein, MCP-1 Protein, MCP1 Protein, SCYA2 Protein, SMC-CF Protein, AI323594 Protein, JE Protein, Scya2 Protein, Sigje Protein, MCP-1A Protein, MCP1A Protein, C-C motif chemokine ligand 2 Protein, chemokine (C-C motif) ligand 2 Protein, C-C motif chemokine 2 Protein, CCL2 Protein, Ccl2 Protein, LOC101120093 Protein
    Background
    Mouse monocyte chemoattractant protein-1 (MCP-1), previously termed JE, is a member of the CC chemokine family and a homolog of human MCP-1. MCP-1 is expressed by a variety of cell types including monocyte/macrophages, endothelial cells and mesangial cells. MCP-1 has chemoattractant activity on monocytes, lymphocytes and basophils but is not active on neutrophils.
    Pathways
    Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, ER-Nucleus Signaling, Unfolded Protein Response, The Global Phosphorylation Landscape of SARS-CoV-2 Infection
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