IL-3 Protein

Catalog No. ABIN1305116

Product Details

Target: IL-3 (Interleukin 3 (IL-3))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: Escherichia coli (E. coli)

Application: ELISA, Functional Studies (Func), Blocking Reagent (BR), Intracellular Flow Cytometry (ICFC)

Brand: BD Pharmingen™

Specificity: Biological Activity: Proliferation is measured using NFS-60 indicator cells, Specific Activity: 0.5 - 5.0 x 10^9 Units/mg, ED50: 2 - 20 pg/ml
Observed dose response: 80 fold.
Note: A Unit is defined as the amount of material required to stimulate a half-maximal response cytokine saturation. Investigators are advised to determine optimal concentrations for individual applications.

Characteristics: Recombinant mouse IL-3 is supplied as a frozen liquid comprised of 0.22 µm sterile-filtered aqueous buffered solution and containing glycerol and 2 mg/ml biotechnology grade, low endotoxin bovine serum albumin, with no preservatives. The recombinant mouse IL-3 was purified by immunoaffinity chromatography and was found to be > 95% pure by SDS-PAGE analysis, and an absorbance assay based on the Beers-Lambert law.

Purification: Immunoaffinity Chromatography
Recombinant mouse IL-3 is supplied as a frozen liquid comprised of 0.22 μm sterile-filtered aqueous buffered solution, glycerol and bovine serum albumin, with no preservatives. Recombinant mouse IL-3 is ≥ 95 % pure as determined by SDS-PAGE, and an absorbance assay based on the Beers-Lambert law.

Purity: ≥ 95 %

Sterility: 0.22 μm filtered

Endotoxin Level: The endotoxin level is ≤ 0.1 ng/µg of IL-3, as measured in a chromogenic LAL assay.

Application Details

Application Notes: ELISA Standard: This recombinant mouse IL-3 is useful as a quantitative standard for measuring mouse IL-3 protein levels in an IL-3 specific sandwich ELISA with the purified MP2-8F8 antibody (Cat. No. 554381) as a capture antibody and the biotinylated MP2-43D11 (Cat. No. 554384) as the detection antibody. To obtain linear standard curves, doubling dilutions of this mouse IL-3 standard from 1800-7.5 pg/ml should be included in each ELISA plate. For specific methodology please visit the protocol sections or the chapter on ELISA in the Immune Function Handbook, both of which are located on our web site, www.bdbiosciences.com.
Note: This ELISA pair is recommended primarily for measuring cytokine from experimental cell culture systems. These ELISA reagents are not recommended for assay of serum or plasma samples. For measuring mouse IL-3 in serum or plasma the BD OptEIA™ Mouse IL-3 ELISA Set (Cat. No. 555228) is specially formulated and recommended.
Ligand Blocking Control for Immunofluorescent Staining of Cytokines: The recombinant mouse IL-3 can be used as a blocking control to demonstrate specificity of IL-3 staining by the PE-labeled MP2-8F8 antibody (Cat. No. 554383). The intracellular cytokine staining technique and use of blocking controls are described in detail by C. Prussin and D. Metcalfe. For specific methodology please visit the protocol sections or the chapter on intracellular staining in the Immune Function Handbook, both of which are located on our web site, www.bdbiosciences.com. Please Note: This application is not routinely tested at BD Biosciences.

Comment:

BD Pharmingen™ Recombinant Mouse IL-3 - Reactivity Ms

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 200 μg/mL

Buffer: Frozen aqueous buffered solution containing BSA and glycerol.

Preservative: Azide free

Handling Advice: Interleukin-3 (IL-3) is a species-specific cytokine which acts on hematopoietic progenitors of every lineage, with the probable exception of cells committed to the lymphoid lineage. It has been referred to as CSF-2 , BPA, megakaryocyte CSF, HCGF and hemopoietin 2. Mouse IL-3 is a 17.2 kD protein containing 140 amino acid residues.

Storage: -80 °C

Storage Comment: Store product at -80°C prior to use or for long term storage of stock solutions. Rapidly thaw and quick-spin product prior to use. Avoid multiple freeze-thaws of product. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

References

Prussin, Metcalfe: "Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies." in: Journal of immunological methods, Vol. 188, Issue 1, pp. 117-28, (1996) (PubMed).

Frendl: "Interleukin 3: from colony-stimulating factor to pluripotent immunoregulatory cytokine." in: International journal of immunopharmacology, Vol. 14, Issue 3, pp. 421-30, (1992) (PubMed).

Weinstein, Ihle, Lavu, Reddy: "Truncation of the c-myb gene by a retroviral integration in an interleukin 3-dependent myeloid leukemia cell line." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 83, Issue 14, pp. 5010-4, (1986) (PubMed).

Urdal, Mochizuki, Conlon, March, Remerowski, Eisenman, Ramthun, Gillis: "Lymphokine purification by reversed-phase high-performance liquid chromatography." in: Journal of chromatography, Vol. 296, pp. 171-9, (1984) (PubMed).

Yokota, Lee, Rennick, Hall, Arai, Mosmann, Nabel, Cantor, Arai: "Isolation and characterization of a mouse cDNA clone that expresses mast-cell growth-factor activity in monkey cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 81, Issue 4, pp. 1070-4, (1984) (PubMed).

Fung, Hapel, Ymer, Cohen, Johnson, Campbell, Young: "Molecular cloning of cDNA for murine interleukin-3." in: Nature, Vol. 307, Issue 5948, pp. 233-7, (1984) (PubMed).

Target Details

Target: IL-3 (Interleukin 3 (IL-3))

Alternative Name: IL-3 (IL-3 Products)

Synonyms: BPA Protein, Csfmu Protein, HCGF Protein, Il-3 Protein, MCGF Protein, PSF Protein, IL-3 Protein, MULTI-CSF Protein, interleukin 3 Protein, interleukin 3 (colony-stimulating factor, multiple) Protein, Il3 Protein, IL3 Protein

Background: Interleukin-3 (IL-3) is a species-specific cytokine which acts on hematopoietic progenitors of every lineage, with the probable exception of cells committed to the lymphoid lineage. It has been referred to as CSF-2 , BPA, megakaryocyte CSF, HCGF and hemopoietin 2. Mouse IL-3 is a 17.2 kD protein containing 140 amino acid residues.

Pathways: JAK-STAT Signaling, Regulation of Carbohydrate Metabolic Process, Autophagy