IL-4 Protein

Catalog No. ABIN1305126

Product Details

Target: IL-4 (IL4) (Interleukin 4 (IL4))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Application: ELISA, Functional Studies (Func)

Brand: BD Pharmingen™

Specificity: Biological Activity: The activity range is measured in a proliferation assay using TF-1 indicator cells
Specific Activity: 0.4 - 5.0 x 10^8 Units /mg
ED50: 20 - 250 pg/ml
Observed dose response relationship: 100 fold A Unit is defined as the amount of material required to stimulate a half-maximal response at cytokine saturation. Since applications vary, each investigator should titrate the reagent to obtain optimal results.

Characteristics: Recombinant human IL-4 is supplied as a frozen liquid comprised of 0.22 µm sterile-filtered aqueous buffered solution, pH 7.2, and containing biotechnology grade, low endotoxin bovine serum albumin, with no preservatives. The human IL-4 was purified by immunoaffinity chromatography and was found to be > 95% pure by SDS-PAGE analysis, and an absorbance assay based on the Beers-Lambert law.

Purification: Immunoaffinity Chromatography

Purity: ≥ 95 %

Sterility: 0.22 μm filtered

Endotoxin Level: The endotoxin level is ≤ 0.1 ng per µg of human IL-4, as measured in a chromogenic LAL assay.

Application Details

Application Notes: ELISA Standard: Human IL-4, Cat. No. 554605, is useful as a quantitative standard for measuring human IL-4 protein levels in an IL-4 specific sandwich ELISA with the purified 8D4-8 antibody (Cat. No. 554515) as a capture antibody and the biotinylated MP4-25D2 (Cat. No. 554483) as the detection antibody. To obtain linear standard curves, doubling dilutions of this human IL-4 standard from ~1000 to 8 pg/ml should be included in each ELISA plate.

Comment:

BD Pharmingen™ Recombinant Human IL-4 - Reactivity Hu

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: Frozen aqueous buffered solution containing BSA.

Preservative: Azide free

Handling Advice: This preparation contains no preservatives, thus it should be handled under aseptic conditions. Rapidly thaw and quick-spin product prior to use. Upon initial thawing, the product should be aliquoted into polypropylene microtubes and frozen at -80°C for future use. Alternatively, the product can be diluted in sterile neutral buffer containing not less than 0.5 - 10 mg/ml carrier protein such as human or bovine albumin, aliquoted and stored at -80°C. For in vitro biological assay use, we recommend carrier-protein concentrations of 1 - 2 mg/ml. For use as an ELISA standard we recommend carrier-protein concentrations of 5 - 10 mg/ml. Failure to add carrier protein or store at indicated temperatures may result in a loss of activity. The product should not be diluted to less than 50 µg/ml for long term storage. Carrier proteins should be pre-screened for possible effects in an appropriate experimental system. Carrier proteins may effect experimental results due to toxicity, high endotoxin levels or possible blocking activity.

Storage: -80 °C

Storage Comment: Store product at -80°C prior to use or for long term storage of stock solutions. Rapidly thaw and quick-spin product prior to use. Avoid multiple freeze-thaws of product. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

References

Paul: "Interleukin-4: a prototypic immunoregulatory lymphokine." in: Blood, Vol. 77, Issue 9, pp. 1859-70, (1991) (PubMed).

Yokota, Otsuka, Mosmann, Banchereau, DeFrance, Blanchard, De Vries, Lee, Arai: "Isolation and characterization of a human interleukin cDNA clone, homologous to mouse B-cell stimulatory factor 1, that expresses B-cell- and T-cell-stimulating activities." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 83, Issue 16, pp. 5894-8, (1986) (PubMed).

Target Details

Target: IL-4 (IL4) (Interleukin 4 (IL4))

Alternative Name: IL-4 (IL4 Products)

Synonyms: IL4 Protein, IL-4 Protein, BCGF-1 Protein, BCGF1 Protein, BSF-1 Protein, BSF1 Protein, Il-4 Protein, Il4e12 Protein, IL2 Protein, interleukin 4 Protein, IL4 Protein, Il4 Protein

Background: Interleukin-4 (IL-4) is a potent multifunctional cytokine which can stimulate the growth, differentiation and maintain the viability of certain subsets of B cells and T cells. Human IL-4 is a 15 kD protein containing 129 amino acid residues. Recombinant human IL-4 (Cat No. 554605) is supplied as a frozen liquid comprised of 0.22 μm sterile-filtered aqueous buffered solution, containing bovine serum albumin, with no preservatives. Recombinant human IL-4 is ≥ 95 % pure as determined by SDS-PAGE, and an absorbance assay based on the Beers-Lambert law. The endotoxin level is ≤0.1 ng per μg of human IL-4, as measured in a chromogenic LAL assay.

Pathways: JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation