Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) (AA 1-125), (full length) protein (GST tag)

Details for Product No. ABIN1310156, Supplier: Log in to see
Protein Name
  • Map1lc3
  • zgc:56434
  • wu:fb60g11
  • map1lc3b
  • MGC76283
  • MAP1LC3B
  • ATG8F
  • LC3B
  • MAP1A/1BLC3
  • MAP1LC3B-a
  • 1010001C15Rik
  • Atg8
  • LC3b
  • Mpl3
  • zbs559
  • microtubule-associated protein 1 light chain 3 beta
  • map1lc3b
  • MAP1LC3B
  • LOC100361973
  • Map1lc3b
Protein Characteristics
AA 1-125, full length
3
1
1
1
1
1
1
1
Origin
Human
7
1
1
1
1
Source
Wheat germ
8
2
1
Protein Type
Recombinant
Purification tag / Conjugate
GST tag
Application
Affinity Purification (AP), Antibody Array (AA), ELISA, Western Blotting (WB)
Options
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Sequence MPSEKTFKQR RTFEQRVEDV RLIREQHPTK IPVIIERYKG EKQLPVLDKT KFLVPDHVNM SELIKIIRRR LQLNANQAFF LLVNGHSMVS VSTPISEVYE SEKDEDGFLY MVYASQETFG MKLSV
Background Full Gene Name: microtubule-associated protein 1 light chain 3 beta
Synonyms: LC3B,MAP1A/1BLC3
Gene ID 81631
Pathways Autophagy
Application Notes Optimal working dilution should be determined by the investigator.
Comment

Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue.

Restrictions For Research Use only
Buffer 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.
Handling Advice Aliquot to avoid repeated freezing and thawing.
Storage -80 °C
Storage Comment Store at -80 °C. Aliquot to avoid repeated freezing and thawing.
Expiry Date 3 months
Product cited in: Koukourakis, Giatromanolaki, Bottini, Cappelletti, Zanotti, Allevi, Strina, Ardine, Milani, Brugnoli, Martinotti, Ferrero, Bertoni, Ferrozzi, Harris, Generali: "Prospective neoadjuvant analysis of PET imaging and mechanisms of resistance to Trastuzumab shows role of HIF1 and autophagy." in: British journal of cancer, Vol. 110, Issue 9, pp. 2209-16, 2014 (PubMed).

Martinet, Schrijvers, Timmermans, Bult, De Meyer: "Immunohistochemical analysis of macroautophagy: recommendations and limitations." in: Autophagy, Vol. 9, Issue 3, pp. 386-402, 2013 (PubMed).

Koukourakis, Giatromanolaki, Zois, Sivridis: "LC3 immunostaining pitfalls." in: Histopathology, Vol. 62, Issue 6, pp. 962-3, 2013 (PubMed).

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