NLRP3 Protein (AA 1-1036) (GST tag)

Catalog No. ABIN1312675

Product Details

Target: NLRP3 (NLR Family, Pyrin Domain Containing 3 (NLRP3))

Protein Type: Recombinant

Protein Characteristics: AA 1-1036

Origin: Human

Source: Wheat germ

Purification tag / Conjugate: This NLRP3 protein is labelled with GST tag.

Application: Western Blotting (WB), ELISA, Affinity Purification (AP), Antibody Array (AA)

Purpose: NLRP3 (Human) Recombinant Protein (P01)

Sequence: MKMASTRCKL ARYLEDLEDV DLKKFKMHLE DYPPQKGCIP LPRGQTEKAD HVDLATLMID FNGEEKAWAM AVWIFAAINR RDLYEKAKRD EPKWGSDNAR VSNPTVICQE DSIEEEWMGL LEYLSRISIC KMKKDYRKKY RKYVRSRFQC IEDRNARLGE SVSLNKRYTR LRLIKEHRSQ QEREQELLAI GKTKTCESPV SPIKMELLFD PDDEHSEPVH TVVFQGAAGI GKTILARKMM LDWASGTLYQ DRFDYLFYIH CREVSLVTQR SLGDLIMSCC PDPNPPIHKI VRKPSRILFL MDGFDELQGA FDEHIGPLCT DWQKAERGDI LLSSLIRKKL LPEASLLITT RPVALEKLQH LLDHPRHVEI LGFSEAKRKE YFFKYFSDEA QARAAFSLIQ ENEVLFTMCF IPLVCWIVCT GLKQQMESGK SLAQTSKTTT AVYVFFLSSL LQPRGGSQEH GLCAHLWGLC SLAADGIWNQ KILFEESDLR NHGLQKADVS AFLRMNLFQK EVDCEKFYSF IHMTFQEFFA AMYYLLEEEK EGRTNVPGSR LKLPSRDVTV LLENYGKFEK GYLIFVVRFL FGLVNQERTS YLEKKLSCKI SQQIRLELLK WIEVKAKAKK LQIQPSQLEL FYCLYEMQEE DFVQRAMDYF PKIEINLSTR MDHMVSSFCI ENCHRVESLS LGFLHNMPKE EEEEEKEGRH LDMVQCVLPS SSHAACSHGL VNSHLTSSFC RGLFSVLSTS QSLTELDLSD NSLGDPGMRV LCETLQHPGC NIRRLWLGRC GLSHECCFDI SLVLSSNQKL VELDLSDNAL GDFGIRLLCV GLKHLLCNLK KLWLVSCCLT SACCQDLASV LSTSHSLTRL YVGENALGDS GVAILCEKAK NPQCNLQKLG LVNSGLTSVC CSALSSVLST NQNLTHLYLR GNTLGDKGIK LLCEGLLHPD CKLQVLELDN CNLTSHCCWD LSTLLTSSQS LRKLSLGNND LGDLGVMMFC EVLKQQSCLL QNLGLSEMYF NYETKSALET LQEEKPELTV VFEPSW

Characteristics: Human NLRP3 full-length ORF ( NP_004886.3, 1 a.a. - 1036 a.a.) recombinant protein with GST-tag at N-terminal.

Purification: in vitro wheat germ expression system

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue.

Restrictions: For Research Use only

Handling

Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -80 °C

Storage Comment: Best use within three months from the date of receipt of this protein.

References

Shi, Wang, Li, Zhan, Tang, Fina, Su, Pratt, Bu, Hildebrand, Lyon, Scott, Quan, Sun, Russell, Arnett, Jurek, Chen, Kravchenko, Mathison, Moresco, Monson, Ulevitch, Beutler: "NLRP3 activation and mitosis are mutually exclusive events coordinated by NEK7, a new inflammasome component." in: Nature immunology, Vol. 17, Issue 3, pp. 250-8, (2016) (PubMed).

He, Varadarajan, Muñoz-Planillo, Burberry, Nakamura, Núñez: "3,4-methylenedioxy-?-nitrostyrene inhibits NLRP3 inflammasome activation by blocking assembly of the inflammasome." in: The Journal of biological chemistry, Vol. 289, Issue 2, pp. 1142-50, (2014) (PubMed).

Cocco, Garella, Di Stilo, Borretto, Stevanato, Giorgis, Marini, Fantozzi, Miglio, Bertinaria: "Electrophilic warhead-based design of compounds preventing NLRP3 inflammasome-dependent pyroptosis." in: Journal of medicinal chemistry, Vol. 57, Issue 24, pp. 10366-82, (2014) (PubMed).

Bruchard, Mignot, Derangère, Chalmin, Chevriaux, Végran, Boireau, Simon, Ryffel, Connat, Kanellopoulos, Martin, Rébé, Apetoh, Ghiringhelli: "Chemotherapy-triggered cathepsin B release in myeloid-derived suppressor cells activates the Nlrp3 inflammasome and promotes tumor growth." in: Nature medicine, Vol. 19, Issue 1, pp. 57-64, (2013) (PubMed).

Lu, Nakamura, Inouye, Li, Tang, Lundbäck, Valdes-Ferrer, Olofsson, Kalb, Roth, Zou, Erlandsson-Harris, Yang, Ting, Wang, Andersson, Antoine, Chavan, Hotamisligil, Tracey: "Novel role of PKR in inflammasome activation and HMGB1 release." in: Nature, Vol. 488, Issue 7413, pp. 670-4, (2012) (PubMed).

Target Details

Target: NLRP3 (NLR Family, Pyrin Domain Containing 3 (NLRP3))

Alternative Name: NLRP3 (NLRP3 Products)

Synonyms: CIAS1 Protein, Nalp3 Protein, avp Protein, cias1 Protein, nalp3 Protein, pypaf1 Protein, agtavprl Protein, AGTAVPRL Protein, AII Protein, AVP Protein, C1orf7 Protein, CLR1.1 Protein, FCAS Protein, FCU Protein, MWS Protein, NALP3 Protein, PYPAF1 Protein, AII/AVP Protein, Cias1 Protein, Mmig1 Protein, Pypaf1 Protein, NLR family pyrin domain containing-like Protein, NLR family pyrin domain containing 3 Protein, NLR family, pyrin domain containing 3 L homeolog Protein, NLR family, pyrin domain containing 3 Protein, NLRPL Protein, NLRP3 Protein, nlrp3.L Protein, Nlrp3 Protein

Background: Full Gene Name: NLR family, pyrin domain containing 3
Synonyms: AGTAVPRL,AII,AII/AVP,AVP,C1orf7,CIAS1,CLR1.1,FCAS,FCU,FLJ95925,MWS,NALP3,PYPAF1

Gene ID: 114548

NCBI Accession: NM_004895

Pathways: Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity, Inflammasome