PDE4D Protein (AA 1-518) (GST tag)
(2 references)-
- Target See all PDE4D Proteins
- PDE4D (phosphodiesterase 4D, cAMP-Specific (PDE4D))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-518
-
Origin
- Human
-
Source
- Wheat germ
- Purification tag / Conjugate
- This PDE4D protein is labelled with GST tag.
- Application
- Western Blotting (WB), ELISA, Affinity Purification (AP), Antibody Array (AA)
- Purpose
- PDE4D (Human) Recombinant Protein (P01)
- Sequence
- MPEANYLLSV SWGYIKFKRM LNRELTHLSE MSRSGNQVSE FISNTFLDKQ HEVEIPSPTQ KEKEKKKRPM SQISGVKKLM HSSSLTNSSI PRFGVKTEQE DVLAKELEDV NKWGLHVFRI AELSGNRPLT VIMHTIFQER DLLKTFKIPV DTLITYLMTL EDHYHADVAY HNNIHAADVV QSTHVLLSTP ALEAVFTDLE ILAAIFASAI HDVDHPGVFN QFLINTNSEL ALMYNDSSVL ENHHLAVGFK LLQEENCDIF QNLTKKQRQS LRKMVIDIVL ATDMSKHMNL LADLKTMVET KKVTSSGVLL LDNYSDRIQV LQNMVHCADL SNPTKPLQLY RQWTDRIMEE FFRQGDRERE RGMEISPMCD KHNASVEKSQ VGFIDYIVHP LWETWADLVH PDAQDILDTL EDNREWYQST IPQSPSPAPD DPEEGRQGQT EKFQFELTLE EDGESDTEKD SGSQVEEDTS CSDSKTLCTQ DSESTEIPLD EQVEEEAVGE EEESQPEACV IDDRSPDT
- Characteristics
- Human PDE4D full-length ORF ( AAH36319, 1 a.a. - 518 a.a.) recombinant protein with GST-tag at N-terminal.
- Purification
- in vitro wheat germ expression system
-
phosphodiesterase 4D, cAMP-Specific (PDE4D) (AA 581-673) protein (GST tag)
PDE4D Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
phosphodiesterase 4D, cAMP-Specific (PDE4D) (AA 1-809) protein (His tag)Crystallography grade PDE4D Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
phosphodiesterase 4D, cAMP-Specific (PDE4D) (AA 1-747) protein (His tag)Crystallography grade PDE4D Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
phosphodiesterase 4D, cAMP-Specific (PDE4D) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)PDE4D Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
-
- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
-
Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue. - Restrictions
- For Research Use only
-
- Buffer
- 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.
- Handling Advice
- Aliquot to avoid repeated freezing and thawing.
- Storage
- -80 °C
- Storage Comment
- Best use within three months from the date of receipt of this protein.
-
-
: "Epac activates the small G proteins Rap1 and Rab3A to achieve exocytosis." in: The Journal of biological chemistry, Vol. 284, Issue 37, pp. 24825-39, (2009) (PubMed).
: "Calcium-induced acrosomal exocytosis requires cAMP acting through a protein kinase A-independent, Epac-mediated pathway." in: The Journal of biological chemistry, Vol. 281, Issue 13, pp. 8656-66, (2006) (PubMed).
-
: "Epac activates the small G proteins Rap1 and Rab3A to achieve exocytosis." in: The Journal of biological chemistry, Vol. 284, Issue 37, pp. 24825-39, (2009) (PubMed).
-
- Target
- PDE4D (phosphodiesterase 4D, cAMP-Specific (PDE4D))
- Alternative Name
- PDE4D (PDE4D Products)
- Background
-
Full Gene Name: phosphodiesterase 4D, cAMP-specific (phosphodiesterase E3 dunce homolog, Drosophila)
Synonyms: DPDE3,HSPDE4D,PDE4DN2,STRK1 - Gene ID
- 5144
- Pathways
- Cellular Response to Molecule of Bacterial Origin, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Regulation of G-Protein Coupled Receptor Protein Signaling
-
