PIAS1 Protein (AA 1-651) (GST tag)
(1 reference)-
- Target See all PIAS1 Proteins
- PIAS1 (Protein Inhibitor of Activated STAT, 1 (PIAS1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-651
-
Origin
- Human
-
Source
- Wheat germ
- Purification tag / Conjugate
- This PIAS1 protein is labelled with GST tag.
- Application
- Western Blotting (WB), ELISA, Affinity Purification (AP), Antibody Array (AA)
- Purpose
- PIAS1 (Human) Recombinant Protein (P01)
- Sequence
- MADSAELKQM VMSLRVSELQ VLLGYAGRNK HGRKHELLTK ALHLLKAGCS PAVQMKIKEL YRRRFPQKIM TPADLSIPNV HSSPMPATLS PSTIPQLTYD GHPASSPLLP VSLLGPKHEL ELPHLTSALH PVHPDIKLQK LPFYDLLDEL IKPTSLASDN SQRFRETCFA FALTPQQVQQ ISSSMDISGT KCDFTVQVQL RFCLSETSCP QEDHFPPNLC VKVNTKPCSL PGYLPPTKNG VEPKRPSRPI NITSLVRLST TVPNTIVVSW TAEIGRNYSM AVYLVKQLSS TVLLQRLRAK GIRNPDHSRA LIKEKLTADP DSEIATTSLR VSLLCPLGKM RLTIPCRALT CSHLQCFDAT LYIQMNEKKP TWVCPVCDKK APYEHLIIDG LFMEILKYCT DCDEIQFKED GTWAPMRSKK EVQEVSASYN GVDGCLSSTL EHQVASHHQS SNKNKKVEVI DLTIDSSSDE EEEEPSAKRT CPSLSPTSPL NNKGILSLPH QASPVSRTPS LPAVDTSYIN TSLIQDYRHP FHMTPMPYDL QGLDFFPFLS GDNQHYNTSL LAAAAAAVSD DQDLLHSSRF FPYTSSQMFL DQLSAGGSTS LPTTNGSSSG SNSSLVSSNS LRESHSHTVT NRSSTDTASI FGIIPDIISL D
- Characteristics
- Human PIAS1 full-length ORF ( NP_057250.1, 1 a.a. - 651 a.a.) recombinant protein with GST-tag at N-terminal.
- Purification
- in vitro wheat germ expression system
-
Protein Inhibitor of Activated STAT, 1 (PIAS1) (AA 543-651) protein (GST tag)
PIAS1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Protein Inhibitor of Activated STAT, 1 (PIAS1) (AA 476-565) protein (GST tag)PIAS1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Protein Inhibitor of Activated STAT, 1 (PIAS1) (AA 2-651) protein (His tag)Crystallography grade PIAS1 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Protein Inhibitor of Activated STAT, 1 (PIAS1) (AA 2-651) protein (His tag)Crystallography grade PIAS1 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
-
- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
-
Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue. - Restrictions
- For Research Use only
-
- Buffer
- 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.
- Handling Advice
- Aliquot to avoid repeated freezing and thawing.
- Storage
- -80 °C
- Storage Comment
- Best use within three months from the date of receipt of this protein.
-
-
: "CREB SUMOylation by the E3 ligase PIAS1 enhances spatial memory." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 34, Issue 29, pp. 9574-89, (2014) (PubMed).
-
: "CREB SUMOylation by the E3 ligase PIAS1 enhances spatial memory." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 34, Issue 29, pp. 9574-89, (2014) (PubMed).
-
- Target
- PIAS1 (Protein Inhibitor of Activated STAT, 1 (PIAS1))
- Alternative Name
- PIAS1 (PIAS1 Products)
- Synonyms
- 2900068C24Rik Protein, Ddxbp1 Protein, GBP Protein, DDXBP1 Protein, GU/RH-II Protein, ZMIZ3 Protein, ddxbp1 Protein, gbp Protein, pias Protein, pias1-A Protein, xPAIS1 Protein, xPIAS1 Protein, zmiz3 Protein, protein inhibitor of activated STAT 1 Protein, protein inhibitor of activated STAT, 1 Protein, protein inhibitor of activated STAT, 1a Protein, protein inhibitor of activated STAT 1 L homeolog Protein, PIAS1 Protein, Pias1 Protein, pias1a Protein, pias1.L Protein
- Background
-
Full Gene Name: protein inhibitor of activated STAT, 1
Synonyms: DDXBP1,GBP,GU/RH-II,MGC141878,MGC141879,ZMIZ3 - Gene ID
- 8554
- NCBI Accession
- NM_016166
- Pathways
- JAK-STAT Signaling, Interferon-gamma Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Muscle Cell Differentiation, Hepatitis C
-
