POLR1E Protein (AA 1-419) (GST tag)
(1 reference)-
- Target See all POLR1E Proteins
- POLR1E (Polymerase (RNA) I Polypeptide E, 53kDa (POLR1E))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-419
-
Origin
- Human
-
Source
- Wheat germ
- Purification tag / Conjugate
- This POLR1E protein is labelled with GST tag.
- Application
- ELISA, Western Blotting (WB), Affinity Purification (AP), Antibody Array (AA)
- Purpose
- PRAF1 (Human) Recombinant Protein (P01)
- Sequence
- MAAEVLPSAR WQYCGAPDGS QRAVLVQFSN GKLQSPGNMR FTLYENKDST NPRKRNQRIL AAETDRLSYV GNNFGTGALK CNTLCRHFVG ILNKTSGQME VYDAELFNMQ PLFSDVSVES ELALESQTKT YREKMDSCIE AFGTTKQKRA LNTRRMNRVG NESLNRAVAK AAETIIDTKG VTALVSDAIH NDLQDDSLYL PPCYDDAAKP EDVYKFEDLL SPAEYEALQS PSEAFRNVTS EEILKMIEEN SHCTFVIEAL KSLPSDVESR DRQARCIWFL DTLIKFRAHR VVKRKSALGP GVPHIINTKL LKHFTCLTYN NGRLRNLISD SMKAKITAYV IILALHIHDF QIDLTVLQRD LKLSEKRMME IAKAMRLKIS KRRVSVAAGS EEDHKLGTLS LPLPPAQTSD RLAKRRKIT
- Characteristics
- Human PRAF1 full-length ORF ( NP_071935.1, 1 a.a. - 419 a.a.) recombinant protein with GST-tag at N-terminal.
- Purification
- in vitro wheat germ expression system
-
Polymerase (RNA) I Polypeptide E, 53kDa (POLR1E) (AA 1-481) protein (His tag)
Crystallography grade POLR1E Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
Polymerase (RNA) I Polypeptide E, 53kDa (POLR1E) (AA 1-482) protein (Strep Tag)Crystallography grade POLR1E Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
Polymerase (RNA) I Polypeptide E, 53kDa (POLR1E) protein (Myc-DYKDDDDK Tag)POLR1E Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
-
- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
-
Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue. - Restrictions
- For Research Use only
-
- Buffer
- 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.
- Handling Advice
- Aliquot to avoid repeated freezing and thawing.
- Storage
- -80 °C
- Storage Comment
- Best use within three months from the date of receipt of this protein.
-
-
: "Repression of rRNA transcription by PARIS contributes to Parkinson's disease." in: Neurobiology of disease, Vol. 73C, pp. 220-228, (2014) (PubMed).
-
: "Repression of rRNA transcription by PARIS contributes to Parkinson's disease." in: Neurobiology of disease, Vol. 73C, pp. 220-228, (2014) (PubMed).
-
- Target
- POLR1E (Polymerase (RNA) I Polypeptide E, 53kDa (POLR1E))
- Alternative Name
- POLR1E (POLR1E Products)
- Synonyms
- 53kDa Protein, AU042259 Protein, D030019D19Rik Protein, Paf53 Protein, Praf1 Protein, PAF53 Protein, PRAF1 Protein, RP11-405L18.3 Protein, RNA polymerase I subunit E Protein, polymerase (RNA) I polypeptide E Protein, polymerase (RNA) I polypeptide E L homeolog Protein, POLR1E Protein, polr1e Protein, Polr1e Protein, polr1e.L Protein
- Background
-
Full Gene Name: polymerase (RNA) I polypeptide E, 53 kDa
Synonyms: FLJ13390,FLJ13970,PAF53,PRAF1,RP11-405L18.3 - Gene ID
- 64425
- NCBI Accession
- NM_022490
-
