RIPK1 Protein (AA 1-671) (GST tag)
(2 references)-
- Target See all RIPK1 Proteins
- RIPK1 (Receptor (TNFRSF)-Interacting serine-threonine Kinase 1 (RIPK1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-671
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Origin
- Human
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Source
- Wheat germ
- Purification tag / Conjugate
- This RIPK1 protein is labelled with GST tag.
- Application
- Western Blotting (WB), ELISA, Affinity Purification (AP), Antibody Array (AA)
- Purpose
- RIPK1 (Human) Recombinant Protein (P01)
- Sequence
- MQPDMSLNVI KMKSSDFLES AELDSGGFGK VSLCFHRTQG LMIMKTVYKG PNCIEHNEAL LEEAKMMNRL RHSRVVKLLG VIIEEGKYSL VMEYMEKGNL MHVLKAEMST PLSVKGRIIL EIIEGMCYLH GKGVIHKDLK PENILVDNDF HIKIADLGLA SFKMWSKLNN EEHNELREVD GTAKKNGGTL YYMAPEHLND VNAKPTEKSD VYSFAVVLWA IFANKEPYEN AICEQQLIMC IKSGNRPDVD DITEYCPREI ISLMKLCWEA NPEARPTFPG IEEKFRPFYL SQLEESVEED VKSLKKEYSN ENAVVKRMQS LQLDCVAVPS SRSNSATEQP GSLHSSQGLG MGPVEESWFA PSLEHPQEEN EPSLQSKLQD EANYHLYGSR MDRQTKQQPR QNVAYNREEE RRRRVSHDPF AQQRPYENFQ NTEGKGTAYS SAASHGNAVH QPSGLTSQPQ VLYQNNGLYS SHGFGTRPLD PGTAGPRVWY RPIPSHMPSL HNIPVPETNY LGNTPTMPFS SLPPTDESIK YTIYNSTGIQ IGAYNYMEIG GTSSSLLDST NTNFKEEPAA KYQAIFDNTT SLTDKHLDPI RENLGKHWKN CARKLGFTQS QIDEIDHDYE RDGLKEKVYQ MLQKWVMREG IKGATVGKLA QALHQCSRID LLSSLIYVSQ N
- Characteristics
- Human RIPK1 full-length ORF (ADR83242.1, 1 a.a. - 671 a.a.) recombinant protein with GST tag at N-terminal.
- Purification
- in vitro wheat germ expression system
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Receptor (TNFRSF)-Interacting serine-threonine Kinase 1 (RIPK1) (AA 292-438) protein (His tag)
RIPK1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Receptor (TNFRSF)-Interacting serine-threonine Kinase 1 (RIPK1) (AA 562-671) protein (GST tag)RIPK1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Receptor (TNFRSF)-Interacting serine-threonine Kinase 1 (RIPK1) (AA 1-671) protein (His tag)Crystallography grade RIPK1 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Receptor (TNFRSF)-Interacting serine-threonine Kinase 1 (RIPK1) (AA 1-656) protein (His tag)Crystallography grade RIPK1 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Receptor (TNFRSF)-Interacting serine-threonine Kinase 1 (RIPK1) (AA 1-257), (full length) protein (His-SUMO Tag)RIPK1 Origin: Drimia maritima (Sea squill) (Charybdis maritima) Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
Receptor (TNFRSF)-Interacting serine-threonine Kinase 1 (RIPK1) (AA 24-247), (partial) protein (His-SUMO Tag)RIPK1 Origin: Bryonia dioica (Red bryony) Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
Receptor (TNFRSF)-Interacting serine-threonine Kinase 1 (RIPK1) (AA 24-269) protein (His tag)RIPK1 Origin: Momordica charantia (Bitter gourd) (Balsam pear) Host: Yeast Recombinant > 90 % SDS
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue - Restrictions
- For Research Use only
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- Buffer
- 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.
- Handling Advice
- Aliquot to avoid repeated freezing and thawing.
- Storage
- -80 °C
- Storage Comment
- Best use within three months from the date of receipt of this protein.
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: "NIK promotes tissue destruction independently of the alternative NF-κB pathway through TNFR1/RIP1-induced apoptosis." in: Cell death and differentiation, Vol. 22, Issue 12, pp. 2020-33, (2015) (PubMed).
: "Cardiac protein kinases: the cardiomyocyte kinome and differential kinase expression in human failing hearts." in: Cardiovascular research, Vol. 108, Issue 1, pp. 87-98, (2015) (PubMed).
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: "NIK promotes tissue destruction independently of the alternative NF-κB pathway through TNFR1/RIP1-induced apoptosis." in: Cell death and differentiation, Vol. 22, Issue 12, pp. 2020-33, (2015) (PubMed).
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- Target
- RIPK1 (Receptor (TNFRSF)-Interacting serine-threonine Kinase 1 (RIPK1))
- Alternative Name
- RIPK1 (RIPK1 Products)
- Synonyms
- RIP Protein, RIP1 Protein, D330015H01Rik Protein, Rinp Protein, Rip1 Protein, rip1 Protein, ripk1 Protein, xRIP1beta Protein, xrip1 Protein, receptor interacting serine/threonine kinase 1 Protein, receptor (TNFRSF)-interacting serine-threonine kinase 1 Protein, receptor interacting serine/threonine kinase 1 L homeolog Protein, RIPK1 Protein, Ripk1 Protein, ripk1.L Protein
- Background
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Full Gene Name: receptor (TNFRSF)-interacting serine-threonine kinase 1
Synonyms: FLJ39204,RIP,RIP1 - Gene ID
- 8737
- Pathways
- NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Activation of Innate immune Response, Inositol Metabolic Process, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Negative Regulation of intrinsic apoptotic Signaling, SARS-CoV-2 Protein Interactome, Ubiquitin Proteasome Pathway
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