Melanoma gp100 Protein (AA 1-661) (GST tag)
(1 reference)-
- Target See all Melanoma gp100 (PMEL) Proteins
- Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-661
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Origin
- Human
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Source
- Wheat germ
- Purification tag / Conjugate
- This Melanoma gp100 protein is labelled with GST tag.
- Application
- Western Blotting (WB), ELISA, Affinity Purification (AP), Antibody Array (AA)
- Purpose
- SILV (Human) Recombinant Protein (P01)
- Sequence
- MDLVLKRCLL HLAVIGALLA VGATKVPRNQ DWLGVSRQLR TKAWNRQLYP EWTEAQRLDC WRGGQVSLKV SNDGPTLIGA NASFSIALNF PGSQKVLPDG QVIWVNNTII NGSQVWGGQP VYPQETDDAC IFPDGGPCPS GSWSQKRSFV YVWKTWGQYW QVLGGPVSGL SIGTGRAMLG THTMEVTVYH RRGSRSYVPL AHSSSAFTIT DQVPFSVSVS QLRALDGGNK HFLRNQPLTF ALQLHDPSGY LAEADLSYTW DFGDSSGTLI SRALVVTHTY LEPGPVTAQV VLQAAIPLTS CGSSPVPGTT DGHRPTAEAP NTTAGQVPTT EVVGTTPGQA PTAEPSGTTS VQVPTTEVIS TAPVQMPTAE STGMTPEKVP VSEVMGTTLA EMSTPEATGM TPAEVSIVVL SGTTAAQVTT TEWVETTARE LPIPEPEGPD ASSIMSTESI TGSLGPLLDG TATLRLVKRQ VPLDCVLYRY GSFSVTLDIV QGIESAEILQ AVPSGEGDAF ELTVSCQGGL PKEACMEISS PGCQPPAQRL CQPVLPSPAC QLVLHQILKG GSGTYCLNVS LADTNSLAVV STQLIMPGQE AGLGQVPLIV GILLVLMAVV LASLIYRRRL MKQDFSVPQL PHSSSHWLRL PRIFCSCPIG ENSPLLSGQQ V
- Characteristics
- Human SILV full-length ORF ( NP_008859.1, 1 a.a. - 661 a.a.) recombinant protein with GST-tag at N-terminal.
- Purification
- in vitro wheat germ expression system
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Premelanosome Protein (PMEL) protein (Fc Tag)
PMEL Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Premelanosome Protein (PMEL) (AA 470-595) protein (His tag)Crystallography grade PMEL Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Premelanosome Protein (PMEL) (AA 437-562) protein (His tag)Crystallography grade PMEL Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Premelanosome Protein (PMEL) (AA 470-661) protein (rho-1D4 tag)Crystallography grade PMEL Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Premelanosome Protein (PMEL) (AA 25-467) protein (rho-1D4 tag)Crystallography grade PMEL Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Premelanosome Protein (PMEL) (AA 25-661) protein (rho-1D4 tag)Crystallography grade PMEL Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Premelanosome Protein (PMEL) (AA 25-626) protein (rho-1D4 tag)Crystallography grade PMEL Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Premelanosome Protein (PMEL) (AA 437-626) protein (rho-1D4 tag)Crystallography grade PMEL Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Premelanosome Protein (PMEL) (AA 25-434) protein (rho-1D4 tag)Crystallography grade PMEL Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue. - Restrictions
- For Research Use only
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- Buffer
- 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.
- Handling Advice
- Aliquot to avoid repeated freezing and thawing.
- Storage
- -80 °C
- Storage Comment
- Best use within three months from the date of receipt of this protein.
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: "BACE2 processes PMEL to form the melanosome amyloid matrix in pigment cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 110, Issue 26, pp. 10658-63, (2013) (PubMed).
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: "BACE2 processes PMEL to form the melanosome amyloid matrix in pigment cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 110, Issue 26, pp. 10658-63, (2013) (PubMed).
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- Target
- Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))
- Alternative Name
- SILV (PMEL Products)
- Synonyms
- D12S53E Protein, ME20 Protein, ME20-M Protein, ME20M Protein, P1 Protein, P100 Protein, PMEL17 Protein, SI Protein, SIL Protein, SILV Protein, gp100 Protein, D10H12S53E Protein, D12S53Eh Protein, Pmel17 Protein, Si Protein, Silv Protein, gp87 Protein, RPE1 Protein, MMP115 Protein, silverb Protein, silver Protein, cb397 Protein, fdv Protein, pmel17 Protein, sb:cb397 Protein, silva Protein, wu:fc11g11 Protein, wu:fj24g11 Protein, zgc:136622 Protein, premelanosome protein Protein, premelanosome protein b Protein, premelanosome protein a Protein, PMEL Protein, Pmel Protein, pmelb Protein, pmela Protein
- Background
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Full Gene Name: silver homolog (mouse)
Synonyms: D12S53E,ME20,PMEL,PMEL17,SI,SIL,gp100 - Gene ID
- 6490
- NCBI Accession
- NM_006928
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