SIRT3 Protein (AA 1-399) (GST tag)
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- Target See all SIRT3 Proteins
- SIRT3 (Sirtuin 3 (SIRT3))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-399
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Origin
- Human
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Source
- Wheat germ
- Purification tag / Conjugate
- This SIRT3 protein is labelled with GST tag.
- Application
- Western Blotting (WB), ELISA, Affinity Purification (AP), Antibody Array (AA)
- Purpose
- SIRT3 (Human) Recombinant Protein (P01)
- Sequence
- MAFWGWRAAA ALRLWGRVVE RVEAGGGVGP FQACGCRLVL GGRDDVSAGL RGSHGARGEP LDPVRPLQRP PRPEVPRAFR RQPRAAAPSF FFSSIKGGRR SISFSVGASS VVGSGGSSDK GKLSLQDVAE LIRARACQRV VVMVGAGIST PSGIPDFRSP GSGLYSNLQQ YDLPYPEAIF ELPFFFHNPK PFFTLAKELY PGNYKPNVTH YFLRLLHDKG LLLRLYTQNI DGLERVSGIP ASKLVEAHGT FASATCTVCQ RPFPGEDIRA DVMADRVPRC PVCTGVVKPD IVFFGEPLPQ RFLLHVVDFP MADLLLILGT SLEVEPFASL TEAVRSSVPR LLINRDLVGP LAWHPRSRDV AQLGDVVHGV ESLVELLGWT EEMRDLVQRE TGKLDGPDK
- Characteristics
- Human SIRT3 full-length ORF ( AAH01042, 1 a.a. - 399 a.a.) recombinant protein with GST-tag at N-terminal.
- Purification
- in vitro wheat germ expression system
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue. - Restrictions
- For Research Use only
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- Buffer
- 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.
- Handling Advice
- Aliquot to avoid repeated freezing and thawing.
- Storage
- -80 °C
- Storage Comment
- Best use within three months from the date of receipt of this protein.
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Mitochondrial SIRT3 and its target glutamate dehydrogenase are altered in follicular cells of women with reduced ovarian reserve or advanced maternal age." in: Human reproduction (Oxford, England), Vol. 29, Issue 7, pp. 1490-9, (2014) (PubMed).
: "
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Mitochondrial SIRT3 and its target glutamate dehydrogenase are altered in follicular cells of women with reduced ovarian reserve or advanced maternal age." in: Human reproduction (Oxford, England), Vol. 29, Issue 7, pp. 1490-9, (2014) (PubMed).
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- Target
- SIRT3 (Sirtuin 3 (SIRT3))
- Alternative Name
- SIRT3 (SIRT3 Products)
- Background
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Full Gene Name: sirtuin (silent mating type information regulation 2 homolog) 3 (S. cerevisiae)
Synonyms: SIR2L3 - Gene ID
- 23410
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