WIF1 Protein (AA 1-379) (GST tag)
(1 reference)-
- Target See all WIF1 Proteins
- WIF1 (WNT Inhibitory Factor 1 (WIF1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-379
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Origin
- Human
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Source
- Wheat germ
- Purification tag / Conjugate
- This WIF1 protein is labelled with GST tag.
- Application
- Western Blotting (WB), ELISA, Antibody Array (AA), Affinity Purification (AP)
- Purpose
- WIF1 (Human) Recombinant Protein (P01)
- Sequence
- MARRSAFPAA ALWLWSILLC LLALRAEAGP PQEESLYLWI DAHQARVLIG FEEDILIVSE GKMAPFTHDF RKAQQRMPAI PVNIHSMNFT WQAAGQAEYF YEFLSLRSLD KGIMADPTVN VPLLGTVPHK ASVVQVGFPC LGKQDGVAAF EVDVIVMNSE GNTILKTPQN AIFFKTCQQA ECPGGCRNGG FCNERRICEC PDGFHGPHCE KALCTPRCMN GGLCVTPGFC ICPPGFYGVN CDKANCSTTC FNGGTCFYPG KCICPPGLEG EQCEISKCPQ PCRNGGKCIG KSKCKCSKGY QGDLCSKPVC EPGCGAHGTC HEPNKCQCQE GWHGRHCNKR YEASLIHALR PAGAQLRQHT PSLKKAEERR DPPESNYIW
- Characteristics
- Human WIF1 full-length ORF ( AAH18037, 1 a.a. - 379 a.a.) recombinant protein with GST-tag at N-terminal.
- Purification
- in vitro wheat germ expression system
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WNT Inhibitory Factor 1 (WIF1) (AA 29-138) protein (GST tag)
WIF1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
WNT Inhibitory Factor 1 (WIF1) protein (His tag)WIF1 Origin: Mouse Host: HEK-293 Cells Recombinant > 93 % as determined by SDS-PAGE
WNT Inhibitory Factor 1 (WIF1) (AA 29-379) protein (His tag)Crystallography grade WIF1 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
WNT Inhibitory Factor 1 (WIF1) protein (Myc-DYKDDDDK Tag)WIF1 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue. - Restrictions
- For Research Use only
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- Buffer
- 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.
- Handling Advice
- Aliquot to avoid repeated freezing and thawing.
- Storage
- -80 °C
- Storage Comment
- Best use within three months from the date of receipt of this protein.
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-
: "Wnt inhibitory factor 1 is epigenetically silenced in human osteosarcoma, and targeted disruption accelerates osteosarcomagenesis in mice." in: The Journal of clinical investigation, Vol. 119, Issue 4, pp. 837-51, (2009) (PubMed).
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: "Wnt inhibitory factor 1 is epigenetically silenced in human osteosarcoma, and targeted disruption accelerates osteosarcomagenesis in mice." in: The Journal of clinical investigation, Vol. 119, Issue 4, pp. 837-51, (2009) (PubMed).
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- Target
- WIF1 (WNT Inhibitory Factor 1 (WIF1))
- Alternative Name
- WIF1 (WIF1 Products)
- Synonyms
- shf Protein, GB10372 Protein, wif-1 Protein, WIF1 Protein, WIF-1 Protein, AW107799 Protein, zgc:110507 Protein, protein shifted Protein, wnt inhibitory factor 1 Protein, WNT inhibitory factor 1 Protein, Wnt inhibitory factor 1 Protein, WNT inhibitory factor 1 S homeolog Protein, LOC552805 Protein, CpipJ_CPIJ003761 Protein, wif1 Protein, WIF1 Protein, Wif1 Protein, LOC100511326 Protein, wif1.S Protein
- Background
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Full Gene Name: WNT inhibitory factor 1
Synonyms: WIF-1 - Gene ID
- 11197
- Pathways
- WNT Signaling, Positive Regulation of fat Cell Differentiation
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