WNT5A Protein (AA 1-380) (GST tag)
(1 reference)-
- Target See all WNT5A Proteins
- WNT5A (Wingless-Type MMTV Integration Site Family, Member 5A (WNT5A))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-380
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Origin
- Human
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Source
- Wheat germ
- Purification tag / Conjugate
- This WNT5A protein is labelled with GST tag.
- Application
- Western Blotting (WB), ELISA, Antibody Array (AA), Affinity Purification (AP)
- Purpose
- WNT5A (Human) Recombinant Protein (P01)
- Sequence
- MKKSIGILSP GVALGMAGSA MSSKFFLVAL AIFFSFAQVV IEANSWWSLG MNNPVQMSEV YIIGAQPLCS QLAGLSQGQK KLCHLYQDHM QYIGEGAKTG IKECQYQFRH RRWNCSTVDN TSVFGRVMQI GSRETAFTYA VSAAGVVNAM SRACREGELS TCGCSRAARP KDLPRDWLWG GCGDNIDYGY RFAKEFVDAR ERERIHAKGS YESARILMNL HNNEAGRRTV YNLADVACKC HGVSGSCSLK TCWLQLADFR KVGDALKEKY DSAAAMRLNS RGKLVQVNSR FNSPTTQDLV YIDPSPDYCV RNESTGSLGT QGRLCNKTSE GMDGCELMCC GRGYDQFKTV QTERCHCKFH WCCYVKCKKC TEIVDQFVCK
- Characteristics
- Human WNT5A full-length ORF ( NP_003383.2, 1 a.a. - 380 a.a.) recombinant protein with GST-tag at N-terminal.
- Purification
- in vitro wheat germ expression system
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Wingless-Type MMTV Integration Site Family, Member 5A (WNT5A) (AA 201-300) protein (GST tag)
WNT5A Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Wingless-Type MMTV Integration Site Family, Member 5A (WNT5A) (AA 62-380) protein (His tag)Crystallography grade WNT5A Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Wingless-Type MMTV Integration Site Family, Member 5A (WNT5A) (AA 62-380) (Active) protein (GST tag)WNT5A Origin: Mouse Host: Escherichia coli (E. coli) > 95 % AcA, CC Active
Wingless-Type MMTV Integration Site Family, Member 5A (WNT5A) protein (Myc-DYKDDDDK Tag)WNT5A Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue. - Restrictions
- For Research Use only
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- Buffer
- 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.
- Handling Advice
- Aliquot to avoid repeated freezing and thawing.
- Storage
- -80 °C
- Storage Comment
- Best use within three months from the date of receipt of this protein.
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: "cGMP-phosphodiesterase 6, transducin and Wnt5a/Frizzled-2-signaling control cGMP and Ca(2+) homeostasis in melanoma cells." in: Cellular and molecular life sciences : CMLS, Vol. 67, Issue 5, pp. 817-28, (2010) (PubMed).
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: "cGMP-phosphodiesterase 6, transducin and Wnt5a/Frizzled-2-signaling control cGMP and Ca(2+) homeostasis in melanoma cells." in: Cellular and molecular life sciences : CMLS, Vol. 67, Issue 5, pp. 817-28, (2010) (PubMed).
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- Target
- WNT5A (Wingless-Type MMTV Integration Site Family, Member 5A (WNT5A))
- Alternative Name
- WNT5A (WNT5A Products)
- Synonyms
- hWNT5A Protein, wnt-5A Protein, wnt5a-B Protein, WNT5A Protein, 8030457G12Rik Protein, Wnt-5a Protein, Wnt family member 5A Protein, Wnt family member 5A S homeolog Protein, wingless-type MMTV integration site family, member 5a Protein, wingless-type MMTV integration site family, member 5A Protein, wingless-related MMTV integration site 5A Protein, Wnt signaling ligand Protein, WNT5A Protein, Wnt5a Protein, wnt5a.S Protein, wnt5a Protein, LOC578814 Protein, wnt5 Protein
- Background
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Full Gene Name: wingless-type MMTV integration site family, member 5A
Synonyms: hWNT5A - Gene ID
- 7474
- NCBI Accession
- NM_003392
- Pathways
- WNT Signaling, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Cell Size, Tube Formation
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