NAMPT Protein (AA 1-491) (His tag)

Catalog No. ABIN1344091

Product Details

Target: NAMPT (Nicotinamide phosphoribosyltransferase (NAMPT))

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: AA 1-491

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This NAMPT protein is labelled with His tag.

Application: SDS-PAGE (SDS)

Cross-Reactivity: Human

Characteristics: Intracellular human Nampt (visfatin/PBEF) (aa 1-491) is fused at the C-terminus to a His-tag.

Purity: >90 % (SDS-PAGE)

Endotoxin Level: <1EU/μg purified protein (LAL test, Lonza).

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Shows adipogenic effects in stimulated differentiating 3T3-L1 cells.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in distilled water.

Concentration: Lot specific

Buffer: Lyophilized. Salt-free.

Storage: 4 °C,-20 °C

Storage Comment: Short Term Storage: +4°C
Long Term Storage: -20°C
Working aliquots are stable for up to 3 months when stored at -20°C.

Expiry Date: 3 months

References

Kover, Tong, Watkins, Clements, Stehno-Bittel, Novikova, Bittel, Kibiryeva, Stuhlsatz, Yan, Ye, Moore: "Expression and regulation of nampt in human islets." in: PLoS ONE, Vol. 8, Issue 3, pp. e58767, (2013) (PubMed).

Yonezawa, Haga, Kobayashi, Takahashi, Obara: "Visfatin is present in bovine mammary epithelial cells, lactating mammary gland and milk, and its expression is regulated by cAMP pathway." in: FEBS letters, Vol. 580, Issue 28-29, pp. 6635-43, (2006) (PubMed).

Target Details

Target: NAMPT (Nicotinamide phosphoribosyltransferase (NAMPT))

Alternative Name: Nampt (Visfatin/PBEF) (NAMPT Products)

Synonyms: 1110035O14Rik Protein, PBEF Protein, PBEF1 Protein, VF Protein, VISFATIN Protein, AI314458 Protein, AI480535 Protein, NAmPRTase Protein, Pbef Protein, Pbef1 Protein, Visfatin Protein, visfatin Protein, pbef Protein, pbef1 Protein, nicotinamide phosphoribosyltransferase Protein, Nicotinamide phosphoribosyltransferase Protein, nicotinamide phosphoribosyltransferase S homeolog Protein, NAMPT Protein, Nampt Protein, nampt Protein, Smon_0680 Protein, Mrub_2845 Protein, Cseg_3704 Protein, BC1002_3997 Protein, Ftrac_2686 Protein, Varpa_5681 Protein, Celal_3900 Protein, Deima_0880 Protein, BC1001_3724 Protein, Celly_1696 Protein, Clole_1542 Protein, Tsp_05934 Protein, nampt.S Protein

Background: Nicotinamide phosphoribosyltransferase (Nampt, pre-B cell colony-enhancing factor, PBEF, Visfatin) is an 52 kDa adipokine secreted by adipose tissue and involved in the biosynthesis of nicotinamide adenine dinucleotide (NAD+). Two forms of Nampt exist, an intracellular form (iNampt) and an extracellular form (eNampt). While the function of iNampt as an essential and rate-limiting NAD+ biosynthetic enzyme is well established, the physiological role of eNampt is still a matter of debate. Nampt has various functions, including the promotion of vascular smooth muscle cell maturation and inhibition of neutrophil apoptosis. It activates insulin receptor and has insulin-mimetic effects, lowering blood glucose and improving insulin sensitivity. The protein is highly expressed in visceral fat and serum levels of the protein correlate with obesity.

Molecular Weight: ~52kDa (SDS-PAGE)

UniProt: P43490