Nicotinamide phosphoribosyltransferase (NAMPT) (AA 1-491) (Active) protein (His tag)

Details for Product No. ABIN1344091, Supplier: Log in to see
Protein Name
  • PBEF1
  • pbef
  • pbef1
  • 1110035O14Rik
  • PBEF
  • VF
  • VISFATIN
  • AI314458
  • AI480535
  • NAmPRTase
  • Pbef
  • Pbef1
  • Visfatin
  • visfatin
  • nicotinamide phosphoribosyltransferase
  • Nicotinamide phosphoribosyltransferase
  • NAMPT
  • nampt
  • Smon_0680
  • Mrub_2845
  • Cseg_3704
  • BC1002_3997
  • Ftrac_2686
  • Varpa_5681
  • Celal_3900
  • Deima_0880
  • BC1001_3724
  • Celly_1696
  • Clole_1542
  • Psed_2146
  • Psed_2158
  • Tsp_05934
  • Nampt
Protein Characteristics
AA 1-491
18
5
2
2
1
Origin
Human
30
12
8
1
1
Source
Escherichia coli (E. coli)
35
4
2
2
2
1
1
Protein Type
Recombinant
Biological Activity
Active
Purification tag / Conjugate
This NAMPT protein is labelled with His tag.
Application
SDS-PAGE (SDS)
Options
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Cross-Reactivity Human
Characteristics Intracellular human Nampt (visfatin/PBEF) (aa 1-491) is fused at the C-terminus to a His-tag.
Purity >90 % (SDS-PAGE)
Endotoxin Level <1EU/μg purified protein (LAL test, Lonza).
Background Nicotinamide phosphoribosyltransferase (Nampt, pre-B cell colony-enhancing factor, PBEF, Visfatin) is an 52 kDa adipokine secreted by adipose tissue and involved in the biosynthesis of nicotinamide adenine dinucleotide (NAD+). Two forms of Nampt exist, an intracellular form (iNampt) and an extracellular form (eNampt). While the function of iNampt as an essential and rate-limiting NAD+ biosynthetic enzyme is well established, the physiological role of eNampt is still a matter of debate. Nampt has various functions, including the promotion of vascular smooth muscle cell maturation and inhibition of neutrophil apoptosis. It activates insulin receptor and has insulin-mimetic effects, lowering blood glucose and improving insulin sensitivity. The protein is highly expressed in visceral fat and serum levels of the protein correlate with obesity.
Molecular Weight ~52kDa (SDS-PAGE)
UniProt P43490
Application Notes Optimal working dilution should be determined by the investigator.
Comment

Shows adipogenic effects in stimulated differentiating 3T3-L1 cells.

Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute in distilled water.
Buffer Lyophilized. Salt-free.
Storage 4 °C,-20 °C
Storage Comment Short Term Storage: +4°C
Long Term Storage: -20°C
Working aliquots are stable for up to 3 months when stored at -20°C.
Expiry Date 3 months
Supplier Images
Western Blotting (WB) image for Nicotinamide phosphoribosyltransferase (NAMPT) (AA 1-491) (Active) protein (His tag) (ABIN1344091) Dimer formation of recombinant Nampt (Visfatin, PBEF) . Purified Nampt (Visfatin, PBE...
Product cited in: Kover, Tong, Watkins, Clements, Stehno-Bittel, Novikova, Bittel, Kibiryeva, Stuhlsatz, Yan, Ye, Moore: "Expression and regulation of nampt in human islets." in: PLoS ONE, Vol. 8, Issue 3, pp. e58767, 2013 (PubMed).

Yonezawa, Haga, Kobayashi, Takahashi, Obara: "Visfatin is present in bovine mammary epithelial cells, lactating mammary gland and milk, and its expression is regulated by cAMP pathway." in: FEBS letters, Vol. 580, Issue 28-29, pp. 6635-43, 2006 (PubMed).

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