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Netrin 1 Protein (NTN1) (AA 25-604) (DYKDDDDK Tag)

NTN1 Origin: Mouse, Human, Rat Host: HEK-293 Cells Recombinant >95 % (SDS-PAGE) SDS Active
Catalog No. ABIN1344382
  • Target See all Netrin 1 (NTN1) Proteins
    Netrin 1 (NTN1)
    Protein Type
    Recombinant
    Biological Activity
    Active
    Protein Characteristics
    AA 25-604
    Origin
    • 6
    • 5
    • 4
    • 2
    Mouse, Human, Rat
    Source
    • 9
    • 2
    • 2
    • 1
    • 1
    HEK-293 Cells
    Purification tag / Conjugate
    This Netrin 1 protein is labelled with DYKDDDDK Tag.
    Application
    SDS-PAGE (SDS)
    Specificity
    Binds to human, mouse and rat UNC5B.
    Cross-Reactivity
    Human, Mouse (Murine), Rat (Rattus)
    Characteristics
    Human netrin-1 (aa 25-604) is fused at the C-terminus to a FLAG®-tag.
    Purity
    >95 % (SDS-PAGE)
    Endotoxin Level
    <0.01EU/μg purified protein (LAL test, Lonza).
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Comment

    Induces pluripotent stem cell (iPS) formation and improves reprogramming efficiency of human and mouse somatic cells by inhibiting apoptosis mediated by the receptors DCC or UNC5B (at 150ng /ml). Induces axon outgrowth.

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Reconstitute with 100 μL sterile distilled water.
    Concentration
    Lot specific
    Buffer
    Lyophilized. Contains PBS plus protein stabilizer. No BSA included. The protein stabilizer shows no interference with protein activity.
    Storage
    4 °C,-20 °C
    Storage Comment
    Short Term Storage: +4°C
    Long Term Storage: -20°C
    Stable for at least 6 months after receipt when stored at -20°C.
    Expiry Date
    6 months
  • Ozmadenci, Féraud, Markossian, Kress, Ducarouge, Gibert, Ge, Durand, Gadot, Plateroti, Bennaceur-Griscelli, Scoazec, Gil, Deng, Bernet, Mehlen, Lavial: "Netrin-1 regulates somatic cell reprogramming and pluripotency maintenance." in: Nature communications, Vol. 6, pp. 7398, (2015) (PubMed).

  • Target
    Netrin 1 (NTN1)
    Alternative Name
    Netrin-1 (NTN1 Products)
    Synonyms
    ntn1-A Protein, Netrin-1 Protein, NTN1 Protein, unc-6 Protein, MGC147073 Protein, NTN1L Protein, AI561871 Protein, BC019633 Protein, netrin-1 Protein, netrin 1 L homeolog Protein, netrin 1 Protein, ntn1.L Protein, NTN1 Protein, ntn1 Protein, Ntn1 Protein
    Background
    Netrin-1 controls guidance of CNS commissural axons and peripheral motor axons. Its association with either DCC or some UNC5 receptors will lead to axon attraction or repulsion, respectively. It also serve as a survival factor via its association with its receptors which prevent the initiation of apoptosis. Netrin-1 is also Involved in tumorigenesis by regulating apoptosis. Netrin-1 promotes atherosclerosis by retaining macrophages in the artery wall. Netrin-1 also governs induced pluripotent stem cell (iPS) formation and improves reprogramming efficiency of human and mouse somatic cells by limiting apoptosis mediated by Netrin-1 receptors DCC or UNC5b.
    Molecular Weight
    ~80kDa (SDS-PAGE)
    UniProt
    O95631
    Pathways
    Regulation of Cell Size
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