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LAG3 Protein (Fc Tag)

LAG3 Origin: Human, Mouse, Monkey Host: CHO Cells Recombinant >99 % (SDS-PAGE) SDS, FACS Active
Catalog No. ABIN1344459
  • Target See all LAG3 Proteins
    LAG3 (Lymphocyte-Activation Gene 3 (LAG3))
    Protein Type
    Recombinant
    Biological Activity
    Active
    Origin
    • 18
    • 10
    • 6
    • 4
    • 1
    • 1
    Human, Mouse, Monkey
    Source
    • 21
    • 8
    • 4
    • 4
    • 2
    • 1
    CHO Cells
    Purification tag / Conjugate
    This LAG3 protein is labelled with Fc Tag.
    Application
    SDS-PAGE (SDS), Flow Cytometry (FACS)
    Specificity
    Binds to human, mouse and monkey MHC class II.
    Cross-Reactivity
    Human, Monkey, Mouse (Murine)
    Characteristics
    The sequence coding for the 4 extracellular Ig-like domains of human LAG-3 (D1-D4) is fused to the Fc portion of human IgG1.
    Purity
    >99 % (SDS-PAGE)
    Endotoxin Level
    <0.1EU/μg purified protein (LAL test, Lonza).
    Top Product
    Discover our top product LAG3 Protein
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Comment

    Flow Cytometry: Detection of MHC class II molecules in combination with fluorescently labeled antibodies to IgG1. Uni-Prot link P18627: LAG-3 (human) http://www.uniprot.org/uniprot/P18627 .Inhibits binding of MAb to LAG-3 (human) to LAG-3. Induces maturation of human dendritic cells.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    Lot specific
    Buffer
    In PBS.
    Storage
    4 °C,-20 °C
    Storage Comment
    Short Term Storage: +4°C
    Long Term Storage: -20°C
    Stable for at least 6 months after receipt when stored at -20°C.
    Expiry Date
    6 months
  • Brignone, Grygar, Marcu, Schäkel, Triebel: "A soluble form of lymphocyte activation gene-3 (IMP321) induces activation of a large range of human effector cytotoxic cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 179, Issue 6, pp. 4202-11, (2007) (PubMed).

    Triebel, Hacene, Pichon: "A soluble lymphocyte activation gene-3 (sLAG-3) protein as a prognostic factor in human breast cancer expressing estrogen or progesterone receptors." in: Cancer letters, Vol. 235, Issue 1, pp. 147-53, (2006) (PubMed).

    Buisson, Triebel: "LAG-3 (CD223) reduces macrophage and dendritic cell differentiation from monocyte precursors." in: Immunology, Vol. 114, Issue 3, pp. 369-74, (2005) (PubMed).

    Andreae, Piras, Burdin, Triebel: "Maturation and activation of dendritic cells induced by lymphocyte activation gene-3 (CD223)." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 168, Issue 8, pp. 3874-80, (2002) (PubMed).

    Huard, Prigent, Pagès, Bruniquel, Triebel: "T cell major histocompatibility complex class II molecules down-regulate CD4+ T cell clone responses following LAG-3 binding." in: European journal of immunology, Vol. 26, Issue 5, pp. 1180-6, (1996) (PubMed).

    Huard, Prigent, Tournier, Bruniquel, Triebel: "CD4/major histocompatibility complex class II interaction analyzed with CD4- and lymphocyte activation gene-3 (LAG-3)-Ig fusion proteins." in: European journal of immunology, Vol. 25, Issue 9, pp. 2718-21, (1995) (PubMed).

  • Target
    LAG3 (Lymphocyte-Activation Gene 3 (LAG3))
    Alternative Name
    LAG-3 (LAG3 Products)
    Synonyms
    CD223 Protein, LAG-3 Protein, Ly66 Protein, lymphocyte activating 3 Protein, lymphocyte-activation gene 3 Protein, LAG3 Protein, Lag3 Protein
    Background
    Lymphocyte activation gene 3 (LAG-3, CD223) plays an important role in negatively regulating T cell proliferation, function and homeostasis. It is required for maximal natural and induced regulatory T cell (Treg) function. LAG-3 is closely related to the T cell co-receptor CD4 and binds to MHC class II molecules but with a significantly higher affinity than CD4.
    Molecular Weight
    ~80kDa (SDS-PAGE)
    UniProt
    P18627
    Pathways
    Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints
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