DDX53 Protein (AA 1-631) (GST tag)

Catalog No. ABIN1351369

Product Details

Target: DDX53 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 53 (DDX53))

Protein Type: Recombinant

Protein Characteristics: AA 1-631

Origin: Human

Source: Wheat germ

Purification tag / Conjugate: This DDX53 protein is labelled with GST tag.

Application: ELISA, Western Blotting (WB), Antibody Array (AA), Affinity Purification (AP)

Purpose: DDX53 (Human) Recombinant Protein (P01)

Sequence: MSHWAPEWKR AEANPRDLGA SWDVRGSRGS GWSGPFGHQG PRAAGSREPP LCFKIKNNMV GVVIGYSGSK IKDLQHSTNT KIQIINGESE AKVRIFGNRE MKAKAKAAIE TLIRKQESYN SESSVDNAAS QTPIGRNLGR NDIVGEAEPL SNWDRIRAAV VECEKRKWAD LPPVKKNFYI ESKATSCMSE MQVINWRKEN FNITCDDLKS GEKRLIPKPT CRFKDAFQQY PDLLKSIIRV GILKPTPIQS QAWPIILQGI DLIVVAQTGT GKTLSYLMPG FIHLDSQPIS REQRNGPGML VLTPTRELAL HVEAECSKYS YKGLKSICIY GGRNRNGQIE DISKGVDIII ATPGRLNDLQ MNNSVNLRSI TYLVIDEADK MLDMEFEPQI RKILLDVRPD RQTVMTSATW PDTVRQLALS YLKDPMIVYV GNLNLVAVNT VKQNIIVTTE KEKRALTQEF VENMSPNDKV IMFVSQKHIA DDLSSDFNIQ GISAESLHGN SEQSDQERAV EDFKSGNIKI LITTDIVSRG LDLNDVTHVY NYDFPRNIDV YVHRVGYIGR TGKTGTSVTL ITQRDSKMAG ELIKILDRAN QSVPEDLVVM AEQYKLNQQK RHRETRSREP GQRRKEFYFL S

Characteristics: Human DDX53 full-length ORF ( AAH67878.1, 1 a.a. - 631 a.a.) recombinant protein with GST-tag at N-terminal.

Purification: in vitro wheat germ expression system

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue.

Restrictions: For Research Use only

Handling

Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -80 °C

Storage Comment: Best use within three months from the date of receipt of this protein.

References

Kim, Park, Kim, Choi, Lee, Lee, Choe, Kim, Jeoung: "miR-200b and cancer/testis antigen CAGE form a feedback loop to regulate the invasion and tumorigenic and angiogenic responses of a cancer cell line to microtubule-targeting drugs." in: The Journal of biological chemistry, Vol. 288, Issue 51, pp. 36502-18, (2013) (PubMed).

Target Details

Target: DDX53 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 53 (DDX53))

Alternative Name: DDX53 (DDX53 Products)

Synonyms: CAGE Protein, CT26 Protein, DEAD-box helicase 53 Protein, DDX53 Protein

Background: Full Gene Name: DEAD (Asp-Glu-Ala-Asp) box polypeptide 53
Synonyms: CAGE

Gene ID: 168400