FOXO3 Protein (AA 1-673) (GST tag)
(1 reference)-
- Target See all FOXO3 Proteins
- FOXO3 (Forkhead Box O3 (FOXO3))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-673
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Origin
- Human
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Source
- Wheat germ
- Purification tag / Conjugate
- This FOXO3 protein is labelled with GST tag.
- Application
- Western Blotting (WB), ELISA, Antibody Array (AA), Affinity Purification (AP)
- Purpose
- FOXO3 (Human) Recombinant Protein (P01)
- Sequence
- MAEAPASPAP LSPLEVELDP EFEPQSRPRS CTWPLQRPEL QASPAKPSGE TAADSMIPEE EDDEDDEDGG GRAGSAMAIG GGGGSGTLGS GLLLEDSARV LAPGGQDPGS GPATAAGGLS GGTQALLQPQ QPLPPPQPGA AGGSGQPRKC SSRRNAWGNL SYADLITRAI ESSPDKRLTL SQIYEWMVRC VPYFKDKGDS NSSAGWKNSI RHNLSLHSRF MRVQNEGTGK SSWWIINPDG GKSGKAPRRR AVSMDNSNKY TKSRGRAAKK KAALQTAPES ADDSPSQLSK WPGSPTSRSS DELDAWTDFR SRTNSNASTV SGRLSPIMAS TELDEVQDDD APLSPMLYSS SASLSPSVSK PCTVELPRLT DMAGTMNLND GLTENLMDDL LDNITLPPSQ PSPTGGLMQR SSSFPYTTKG SGLGSPTSSF NSTVFGPSSL NSLRQSPMQT IQENKPATFS SMSHYGNQTL QDLLTSDSLS HSDVMMTQSD PLMSQASTAV SAQNSRRNVM LRNDPMMSFA AQPNQGSLVN QNLLHHQHQT QGALGGSRAL SNSVSNMGLS ESSSLGSAKH QQQSPVSQSM QTLSDSLSGS SLYSTSANLP VMGHEKFPSD LDLDMFNGSL ECDMESIIRS ELMDADGLDF NFDSLISTQN VVGLNVGNFT GAKQASSQSW VPG
- Characteristics
- Human FOXO3 full-length ORF ( AAH20227.1, 1 a.a. - 673 a.a.) recombinant protein with GST-tag at N-terminal.
- Purification
- in vitro wheat germ expression system
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Forkhead Box O3 (FOXO3) (AA 478-673) protein (His-IF2DI Tag)
FOXO3 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Forkhead Box O3 (FOXO3) (AA 361-460) protein (GST tag)FOXO3 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Forkhead Box O3 (FOXO3) (AA 1-673) protein (His tag)Crystallography grade FOXO3 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Forkhead Box O3 (FOXO3) (AA 1-672) protein (His tag)Crystallography grade FOXO3 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Forkhead Box O3 (FOXO3) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)FOXO3 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Forkhead Box O3 (FOXO3) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)FOXO3 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue. - Restrictions
- For Research Use only
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- Buffer
- 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.
- Handling Advice
- Aliquot to avoid repeated freezing and thawing.
- Storage
- -80 °C
- Storage Comment
- Best use within three months from the date of receipt of this protein.
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: "FOXO3 is a glucocorticoid receptor target and regulates LKB1 and its own expression based on cellular AMP levels via a positive autoregulatory loop." in: PLoS ONE, Vol. 7, Issue 7, pp. e42166, (2012) (PubMed).
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: "FOXO3 is a glucocorticoid receptor target and regulates LKB1 and its own expression based on cellular AMP levels via a positive autoregulatory loop." in: PLoS ONE, Vol. 7, Issue 7, pp. e42166, (2012) (PubMed).
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- Target
- FOXO3 (Forkhead Box O3 (FOXO3))
- Alternative Name
- FOXO3 (FOXO3 Products)
- Synonyms
- AF6q21 Protein, FKHRL1 Protein, FKHRL1P2 Protein, FOXO2 Protein, FOXO3A Protein, zgc:92176 Protein, wu:fb50g02 Protein, wu:fi33f07 Protein, Fkhrl1 Protein, Foxo3a Protein, FoxO3A Protein, 1110048B16Rik Protein, 2010203A17Rik Protein, C76856 Protein, Fkhr2 Protein, FOXO-3 Protein, FOXO3 Protein, foxo3a Protein, xfoxo3 Protein, foxO3 Protein, foxO Protein, fkhr Protein, foxo5 Protein, wu:fj65a12 Protein, zFkhr Protein, forkhead box O3 Protein, forkhead box O3A Protein, forkhead box O3 L homeolog Protein, forkhead box O3b Protein, FOXO3 Protein, foxo3a Protein, Foxo3 Protein, foxo3.L Protein, foxo3 Protein, foxO3 Protein, foxo3b Protein
- Background
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Full Gene Name: forkhead box O3
Synonyms: AF6q21,DKFZp781A0677,FKHRL1,FKHRL1P2,FOXO2,FOXO3A,MGC12739,MGC31925 - Gene ID
- 2309
- Pathways
- Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Carbohydrate Homeostasis
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