DBT Protein (AA 62-482) (His tag)

Catalog No. ABIN1459612

Product Details

Target: DBT (Dihydrolipoamide Branched Chain Transacylase E2 (DBT))

Protein Type: Recombinant

Protein Characteristics: AA 62-482

Origin: Cow

Source: Yeast

Purification tag / Conjugate: This DBT protein is labelled with His tag.

Application: ELISA

Sequence: GQIVQFKLS DIGEGIREVT VKEWYVKEGD TVSQFDSICE VQSDKASVTI TSRYDGVIKK LYYNLDDTAY VGKPLVDIET EALKDSEEDV VETPAVSHDE HTHQEIKGQK TLATPAVRRL AMENNIKLSE VIGSGKDGRI LKEDILNYLE KQTGAILPPS PKAEIMPPPP KPKDRTIPIP ISKPPVFIGK DRTEPVKGFH KAMVKTMSAA LKIPHFGYCD EVDLTELVKL REELKPIAFA RGIKLSFMPF FLKAASLGLL QFPILNASVD ENCQNITYKA SHNIGIAMDT EQGLIVPNVK NVQIRSIFEI ATELNRLQKL GSAGQLSTND LIGGTFTLSN IGSIGGTYAK PVILPPEVAI GALGTIKALP RFNEKGEVCK AQIMNVSWSA DHRIIDGATV SRFSNLWKSY LENPAFMLLD LK

Specificity: Bos taurus (Bovine)

Characteristics: Please inquire if you are interested in this recombinant protein expressed in E. coli, mammalien cells or by baculovirus infection. Be aware about differences in price and lead time.

Purity: > 90 %

Application Details

Comment:

The yeast protein expression system is the most economical and efficient eukaryotic system for secretion and intracellular expression. A protein expressed by the mammalian cell system is of very high-quality and close to the natural protein. But the low expression level, the high cost of medium and the culture conditions restrict the promotion of mammalian cell expression systems. The yeast protein expression system serve as a eukaryotic system integrate the advantages of the mammalian cell expression system. A protein expressed by yeast system could be modificated such as glycosylation, acylation, phosphorylation and so on to ensure the native protein conformation. It can be used to produce protein material with high added value that is very close to the natural protein. Our proteins produced by yeast expression system has been used as raw materials for downstream preparation of monoclonal antibodies.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: 0.2-2 mg/mL

Buffer: Tris-based buffer, 50 % glycerol

Handling Advice: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week

Storage: -20 °C

Storage Comment: Store at -20 °C, for extended storage, conserve at -20 °C or -80 °C.

Target Details

Target: DBT (Dihydrolipoamide Branched Chain Transacylase E2 (DBT))

Alternative Name: Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial (DBT) (DBT Products)

Synonyms: BCATE2 Protein, BCKAD-E2 Protein, BCKADE2 Protein, E2 Protein, E2B Protein, D3Wsu60e Protein, im:7147214 Protein, zgc:103768 Protein, E2b Protein, dihydrolipoamide branched chain transacylase E2 Protein, hypothetical protein Protein, dihydrolipoamide branched chain transacylase E2 L homeolog Protein, DBT Protein, dbt Protein, CpipJ_CPIJ006326 Protein, BDBG_05874 Protein, NAEGRDRAFT_78509 Protein, VDBG_04820 Protein, PGTG_17722 Protein, Dbt Protein, dbt.L Protein

Target Type: Viral Protein

Background: Recommended name: Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial.
EC= 2.3.1.168.
Alternative name(s): Branched-chain alpha-keto acid dehydrogenase complex component E2.
Short name= BCKAD-E2.
Short name= BCKADE2 Dihydrolipoamide acetyltransferase component of branched-chain alpha-keto acid dehydrogenase complex Dihydrolipoamide branched chain transacylase Dihydrolipoyllysine-residue (2-methylpropanoyl)transferase

UniProt: P11181