Glucosamine (UDP-N-Acetyl)-2-Epimerase/N-Acetylmannosamine Kinase (GNE) (AA 1-722) protein (His tag)

Details for Product No. ABIN1475568, Supplier: Log in to see
Protein Name
  • DMRV
  • GLCNE
  • IBM2
  • UAE1
  • zgc:77657
  • GNE
  • MGC145505
  • NM
  • Uae1
  • 2310066H07Rik
  • glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase
  • GNE
  • gne
  • Gne
Protein Characteristics
AA 1-722
3
1
1
1
1
Origin
Rat (Rattus)
4
1
Source
Yeast
2
2
1
Protein Type
Recombinant
Purification tag / Conjugate
This GNE protein is labelled with His tag.
Application
ELISA
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Sequence MEKNGNNRKL RVCVATCNRA DYSKLAPIMF GIKTEPAFFE LDVVVLGSHL IDDYGNTYRM IEQDDFDINT RLHTIVRGED EAAMVESVGL ALVKLPDVLN RLKPDIMIVH GDRFDALALA TSAALMNIRI LHIEGGEVSG TIDDSIRHAI TKLAHYHVCC TRSAEQHLIS MCEDHDRILL AGCPSYDKLL SAKNKDYMSI IRMWLGDDVK CKDYIVALQH PVTTDIKHSI KMFELTLDAL ISFNKRTLVL FPNIDAGSKE MVRVMRKKGI EHHPNFRAVK HVPFDQFIQL VAHAGCMIGN SSCGVREVGA FGTPVINLGT RQIGRETGEN VLHVRDADTQ DKILQALHLQ FGKQYPCSKI YGDGNAVPRI LKFLKSIDLQ EPLQKKFCFP PVKENISQDI DHILETLSAL AVDLGGTNLR VAIVSMKGEI VKKYTQFNPK TYEERISLIL QMCVEAAAEA VKLNCRILGV GISTGGRVNP QEGVVLHSTK LIQEWNSVDL RTPLSDTLHL PVWVDNDGNC AAMAERKFGQ GKGQENFVTL ITGTGIGGGI IHQHELIHGS SFCAAELGHL VVSLDGPDCS CGSHGCIEAY ASGMALQREA KKLHDEDLLL VEGMSVPKDE AVGALHLIQA AKLGNVKAQS ILRTAGTALG LGVVNILHTM NPSLVILSGV LASHYIHIVR DVIRQQALSS VQDVDVVVSD LVDPALLGAA SMVLDYTTRR IH
Specificity Rattus norvegicus (Rat)
Characteristics Please inquire if you are interested in this recombinant protein expressed in E. coli, mammalien cells or by baculovirus infection. Be aware about differences in price and lead time.
Purity > 90 %
Background Recommended name: Bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase.
Alternative name(s): UDP-GlcNAc-2-epimerase/ManAc kinase Including the following 2 domains: UDP-N-acetylglucosamine 2-epimerase.
EC= 5.1.3.14.
Alternative name(s): UDP-GlcNAc-2-epimerase Uridine diphosphate-N-acetylglucosamine-2-epimerase N-acetylmannosamine kinase.
EC= 2.7.1.60.
Alternative name(s): ManAc kinase
UniProt O35826
Comment

The yeast protein expression system is the most economical and efficient eukaryotic system for secretion and intracellular expression. A protein expressed by the mammalian cell system is of very high-quality and close to the natural protein. But the low expression level, the high cost of medium and the culture conditions restrict the promotion of mammalian cell expression systems. The yeast protein expression system serve as a eukaryotic system integrate the advantages of the mammalian cell expression system. A protein expressed by yeast system could be modificated such as glycosylation, acylation, phosphorylation and so on to ensure the native protein conformation. It can be used to produce protein material with high added value that is very close to the natural protein. Our proteins produced by yeast expression system has been used as raw materials for downstream preparation of monoclonal antibodies.

Restrictions For Research Use only
Format Lyophilized
Concentration 0.2-2 mg/mL
Buffer Tris-based buffer, 50 % glycerol
Handling Advice Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week
Storage -20 °C
Storage Comment Store at -20 °C, for extended storage, conserve at -20 °C or -80 °C.
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