CYP7A1 Protein (AA 1-503) (His tag)

Catalog No. ABIN1476025

Product Details

Target: CYP7A1 (Cytochrome P450, Family 7, Subfamily A, Polypeptide 1 (CYP7A1))

Protein Type: Recombinant

Protein Characteristics: AA 1-503

Origin: Rat

Source: Yeast

Purification tag / Conjugate: This CYP7A1 protein is labelled with His tag.

Application: ELISA

Sequence: MMTISLIWGI AVLVSCCIWF IVGIRRRKAG EPPLENGLIP YLGCALKFGS NPLEFLRANQ RKHGHVFTCK LMGKYVHFIT NSLSYHKVLC HGKYFDWKKF HYTTSAKAFG HRSIDPNDGN TTENINNTFT KTLQGDALCS LSEAMMQNLQ SVMRPPGLPK SKSNAWVTEG MYAFCYRVMF EAGYLTLFGR DISKTDTQKA LILNNLDNFK QFDQVFPALV AGLPIHLFKT AHKAREKLAE GLKHKNLCVR DQVSELIRLR MFLNDTLSTF DDMEKAKTHL AILWASQANT IPATFWSLFQ MIRSPEAMKA ASEEVSGALQ SAGQELSSGG SAIYLDQVQL NDLPVLDSII KEALRLSSAS LNIRTAKEDF TLHLEDGSYN IRKDDMIALY PQLMHLDPEI YPDPLTFKYD RYLDESGKAK TTFYSNGNKL KCFYMPFGSG ATICPGRLFA VQEIKQFLIL MLSCFELEFV ESQVKCPPLD QSRAGLGILP PLHDIEFKYK LKH

Specificity: Rattus norvegicus (Rat)

Characteristics: Please inquire if you are interested in this recombinant protein expressed in E. coli, mammalien cells or by baculovirus infection. Be aware about differences in price and lead time.

Purity: > 90 %

Application Details

Comment:

The yeast protein expression system is the most economical and efficient eukaryotic system for secretion and intracellular expression. A protein expressed by the mammalian cell system is of very high-quality and close to the natural protein. But the low expression level, the high cost of medium and the culture conditions restrict the promotion of mammalian cell expression systems. The yeast protein expression system serve as a eukaryotic system integrate the advantages of the mammalian cell expression system. A protein expressed by yeast system could be modificated such as glycosylation, acylation, phosphorylation and so on to ensure the native protein conformation. It can be used to produce protein material with high added value that is very close to the natural protein. Our proteins produced by yeast expression system has been used as raw materials for downstream preparation of monoclonal antibodies.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: 0.2-2 mg/mL

Buffer: Tris-based buffer, 50 % glycerol

Handling Advice: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week

Storage: -20 °C

Storage Comment: Store at -20 °C, for extended storage, conserve at -20 °C or -80 °C.

Target Details

Target: CYP7A1 (Cytochrome P450, Family 7, Subfamily A, Polypeptide 1 (CYP7A1))

Alternative Name: Cholesterol 7-alpha-monooxygenase (Cyp7a1) (CYP7A1 Products)

Synonyms: CP7A Protein, CYP7 Protein, CYPVII Protein, CHAP Protein, CYP7S1 Protein, cyp7a1a Protein, zgc:63920 Protein, cytochrome P450 family 7 subfamily A member 1 Protein, cytochrome P450, family 7, subfamily a, polypeptide 1 Protein, cytochrome P450, family 7, subfamily A, polypeptide 1 Protein, cholesterol 7-alpha-hydroxylase Protein, CYP7A1 Protein, Cyp7a1 Protein, cyp7a1 Protein

Background: Recommended name: Cholesterol 7-alpha-monooxygenase.
EC= 1.14.13.17.
Alternative name(s): CYPVII Cholesterol 7-alpha-hydroxylase Cytochrome P450 7A1

UniProt: P18125

Pathways: Steroid Hormone Biosynthesis, Carbohydrate Homeostasis, Regulation of Lipid Metabolism by PPARalpha