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Protein L Protein (Biotin)

Origin: Peptostreptococcus magnus WB, DB, IHC, ELISA
Catalog No. ABIN1536530
  • Target See all Protein L Proteins
    Protein L
    Origin
    • 4
    • 1
    Peptostreptococcus magnus
    Host
    Please inquire
    Purification tag / Conjugate
    This Protein L protein is labelled with Biotin.
    Application
    Western Blotting (WB), Dot Blot (DB), Immunohistochemistry (IHC), ELISA
    Top Product
    Discover our top product Protein L Protein
  • Application Notes
    Working concentrations for specific applications should be determined by titration assay. The chart below may serve as a useful guideline, but primary antibody, temperature, incubation time, the amount of antigen, and the sensitivity of the detection methods can all affect the ideal working concentration. Dot blot: 1:2,000-1:20,000 Western blot: 1:2,000-1:20,000 ELISA: 1:20,000-1:100,000 Other: user-optimized
    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Reconstitute the lyophilized product with deionized water (or equivalent) to the final concentration of 1.0 mg/ml.
    Concentration
    1 mg/ml
    Buffer
    PBS (pH 7.4) containing 10 mg/ml BSA and 0.02% sodium azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    The product is stable in lyophilized form if stored at -20°C or below. The reconstituted product can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
  • Hillerdal, Ramachandran, Leja, Essand: "Systemic treatment with CAR-engineered T cells against PSCA delays subcutaneous tumor growth and prolongs survival of mice." in: BMC cancer, Vol. 14, pp. 30, (2014) (PubMed).

    Abate-Daga, Lagisetty, Tran, Zheng, Gattinoni, Yu, Burns, Miermont, Teper, Rudloff, Restifo, Feldman, Rosenberg, Morgan: "A novel chimeric antigen receptor against prostate stem cell antigen mediates tumor destruction in a humanized mouse model of pancreatic cancer." in: Human gene therapy, Vol. 25, Issue 12, pp. 1003-12, (2014) (PubMed).

    Lanitis, Poussin, Hagemann, Coukos, Sandaltzopoulos, Scholler, Powell: "Redirected antitumor activity of primary human lymphocytes transduced with a fully human anti-mesothelin chimeric receptor." in: Molecular therapy : the journal of the American Society of Gene Therapy, Vol. 20, Issue 3, pp. 633-43, (2012) (PubMed).

    Morgan, Johnson, Davis, Zheng, Woolard, Reap, Feldman, Chinnasamy, Kuan, Song, Zhang, Fine, Rosenberg: "Recognition of glioma stem cells by genetically modified T cells targeting EGFRvIII and development of adoptive cell therapy for glioma." in: Human gene therapy, Vol. 23, Issue 10, pp. 1043-53, (2012) (PubMed).

  • Target
    Protein L
    Abstract
    Protein L Products
    Background
    Protein L is a cell surface protein from Peptostreptoccocus magnus that binds to the variable light chains (kappa chain) of immunoglobulins without interfering with antigen binding. In contrast to IgG-binding proteins, such as protein A and protein G, protein L can be used for the detection and purification of mammalian kappa light chain antibodies of all classes. Biotin-Protein L, which is produced by covalently linking biotin to protein L, can be used in immunodetections, such as immunoblotting (western or dot blot), ELISA, and immunohistochemistry.
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