IgE Protein

Details for Product No. ABIN1583007, Supplier: Log in to see
Protein Name
  • IGH2
  • IgE
  • Gm900
  • immunoglobulin heavy chain (epsilon polypeptide)
  • immunoglobulin heavy constant epsilon
  • Immunoglobulin heavy constant epsilon
  • Ighe
  • IGHE
Origin
Human
29
6
1
Source
Human
29
6
1
Clonality
Polyclonal
Application
Isotype Control (IsoC), ELISA
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Characteristics This product is purified human IgE with kappa light chains produced in vitro from a hybridoma. Plasma IgE concentrations are low in normal patients. Elevated IgE levels are found in patients presenting severe allergic reactions and parasite infections. Original material was obtained from a healthy donor tested negative for HIV, HCV and hepatitis B by using US FDA-approved tests. Purity of human IgE is > 98 % by SDS-PAGE analysis. There is no contamination by antibodies of other isotypes because of the cell line origin. Contaminants from foetal bovine serum were removed by Protein-L affinity chromatography. The material must be handled as potentially infectious as all human material. The product can be used as a control.
This is a non-immune hybridoma.
Purification Affinity Chromatography on Protein L
Purity > 98 % by SDS-PAGE
Research Area Immunology, Secondary Antibodies
Application Notes Purified human IgE with kappa light chain is produced in vitro from a monoclonal hybridoma. Original material was obtained from a healthy donor tested negative for HIV, HCV and hepatitis B by using US FDA-approved tests.
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer 0.1 mg IgE in 0.1 mL of PBS pH 7.4. No sodium azide.
Preservative Sodium azide
Precaution of Use Warning: This product contains sodium azide, which has been classified as Xn (Harmful), in European Directive 67/548/EEC in the concentration range of 0.1 - 1.0 %. When disposing of this reagent through lead or copper plumbing, flush with copious volumes of water to prevent azide build-up in drains.
Handling Advice Minimize freeze-thaw cycles. The material must be handled as potentially infectious as all human material.
Storage -20 °C
Expiry Date 12 months
Supplier Images
 image for IgE protein (ABIN1583007) Quality control of human IgE preparation from hybridoma (lane 2) vs. IgE sample purif...
Product cited in: Palaniyandi, Tomei, Li, Conrad, Zhu: "CD23-dependent transcytosis of IgE and immune complex across the polarized human respiratory epithelial cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 186, Issue 6, pp. 3484-96, 2011 (PubMed).

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