IgE protein (Biotin)

Details for Product No. ABIN1583010, Supplier: Log in to see
Protein Name
  • IGH2
  • IgE
  • Gm900
  • immunoglobulin heavy chain (epsilon polypeptide)
  • immunoglobulin heavy constant epsilon
  • Immunoglobulin heavy constant epsilon
  • Ighe
  • IGHE
Origin
Human
29
6
1
Source
Human
29
6
1
Clonality
Polyclonal
Purification tag / Conjugate
Biotin
Application
Isotype Control (IsoC), ELISA
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Characteristics This product is purified human IgE with kappa light chains produced in vitro from a hybridoma. Plasma IgE concentrations are low in normal patients. Elevated IgE levels are found in patients presenting severe allergic reactions and parasite infections. Original material was obtained from a healthy donor tested negative for HIV, HCV and hepatitis B by using US FDA-approved tests. Purity of human IgE is > 98 % by SDS-PAGE analysis. There is no contamination by antibodies of other isotypes because of the cell line origin. Contaminants from foetal bovine serum were removed by Protein-L affinity chromatography. The material must be handled as potentially infectious as all human material. The product can be used as a control.
This is a non-immune hybridoma.
Purification Affinity Chromatography on Protein L
Purity > 98 % by SDS-PAGE
Research Area Immunology, Secondary Antibodies
Application Notes Purified human IgE with kappa light chain is produced in vitro from a monoclonal hybridoma. Original material was obtained from a healthy donor tested negative for HIV, HCV and hepatitis B by using US FDA-approved tests.
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer 0.1 mg IgE in 0.1 mL of PBS pH 7.4, 0.1 % BSA with 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use Warning: This product contains sodium azide, which has been classified as Xn (Harmful), in European Directive 67/548/EEC in the concentration range of 0.1 - 1.0 %. When disposing of this reagent through lead or copper plumbing, flush with copious volumes of water to prevent azide build-up in drains.
Handling Advice Minimize freeze-thaw cycles. The material must be handled as potentially infectious as all human material.
Storage -20 °C
Expiry Date 12 months
Supplier Images
 image for IgE protein (Biotin) (ABIN1583010) Quality control of human IgE preparation from hybridoma (lane 2) vs. IgE sample purif...
ELISA image for IgE protein (Biotin) (ABIN1583010) IgE protein (Biotin) (Image 2)
Product cited in: Serra-Pages, Olivera, Torres, Picado, de Mora, Rivera: "E-prostanoid 2 receptors dampen mast cell degranulation via cAMP/PKA-mediated suppression of IgE-dependent signaling." in: Journal of leukocyte biology, Vol. 92, Issue 6, pp. 1155-65, 2012 (PubMed).

Ainsua-Enrich, Alvarez-Errico, Gilfillan, Picado, Sayós, Rivera, Martín: "The adaptor 3BP2 is required for early and late events in FcεRI signaling in human mast cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 189, Issue 6, pp. 2727-34, 2012 (PubMed).

Janson, Kasahara, Prendergast, Colicelli: "RIN3 is a negative regulator of mast cell responses to SCF." in: PLoS ONE, Vol. 7, Issue 11, pp. e49615, 2012 (PubMed).

Álvarez-Errico, Oliver-Vila, Ainsua-Enrich, Gilfillan, Picado, Sayós, Martín: "CD84 negatively regulates IgE high-affinity receptor signaling in human mast cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 187, Issue 11, pp. 5577-86, 2011 (PubMed).

Charles, Hardwick, Daugas, Illei, Rivera: "Basophils and the T helper 2 environment can promote the development of lupus nephritis." in: Nature medicine, Vol. 16, Issue 6, pp. 701-7, 2010 (PubMed).