Cathepsin B Protein (CTSB) (AA 82-333, partial) (GST tag)

Catalog No. ABIN1608115

Product Details

Target: Cathepsin B (CTSB)

Protein Type: Recombinant

Protein Characteristics: AA 82-333, partial

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This Cathepsin B protein is labelled with GST tag.

Application: ELISA

Sequence: ASFDAREQWP QCPTIKEIRD QGSCGSCWAF GAVEAISDRI CIHTNAHVSV EVSAEDLLTC CGSMCGDGCN GGYPAEAWNF WTRKGLVSGG LYESHVGCRP YSIPPCEHHV NGSRPPCTGE GDTPKCSKIC EPGYSPTYKQ DKHYGYNSYS VSNSEKDIMA EIYKNGPVEG AFSVYSDFLL YKSGVYQHVT GEMMGGHAIR ILGWGVENGT PYWLVANSWN TDWGDNGFFK ILRGQDHCGI ESEVVAGIPR TD

Characteristics: Please inquire if you are interested in this recombinant protein expressed in E. coli, mammalien cells or by baculovirus infection. Be aware about differences in price and lead time.

Purity: 90 %

Application Details

Comment:

The yeast protein expression system is the most economical and efficient eukaryotic system for secretion and intracellular expression. A protein expressed by the mammalian cell system is of very high-quality and close to the natural protein. But the low expression level, the high cost of medium and the culture conditions restrict the promotion of mammalian cell expression systems. The yeast protein expression system serve as a eukaryotic system integrate the advantages of the mammalian cell expression system. A protein expressed by yeast system could be modificated such as glycosylation, acylation, phosphorylation and so on to ensure the native protein conformation. It can be used to produce protein material with high added value that is very close to the natural protein. Our proteins produced by yeast expression system has been used as raw materials for downstream preparation of monoclonal antibodies.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: 0.2-2 mg/mL

Buffer: Tris-based buffer, 50 % glycerol

Handling Advice: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week

Storage: -20 °C

Storage Comment: Store at -20 °C for extended storage, conserve at -20 °C or -80 °C

References

Cao, Taggart, Berquin, Moin, Fong, Sloane: "Human gastric adenocarcinoma cathepsin B: isolation and sequencing of full-length cDNAs and polymorphisms of the gene." in: Gene, Vol. 139, Issue 2, pp. 163-9, (1994) (PubMed).

Chan, San Segundo, McCormick, Steiner: "Nucleotide and predicted amino acid sequences of cloned human and mouse preprocathepsin B cDNAs." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 83, Issue 20, pp. 7721-5, (1986) (PubMed).

Target Details

Target: Cathepsin B (CTSB)

Alternative Name: Cathepsin B protein (CTSB Products)

Synonyms: APPS Protein, CPSB Protein, CB Protein, apps Protein, cpsb Protein, LOC692390 Protein, CTSB Protein, cathepsin B Protein, cathepsin B S homeolog Protein, CTSB Protein, Ctsb Protein, ctsb.S Protein, LOC692390 Protein

Background: Thiol protease which is believed to participate in intracellular degradation and turnover of proteins. Has also been implicated in tumor invasion and metastasis.

Molecular Weight: 55 kD

UniProt: P07858

Pathways: Activation of Innate immune Response, Toll-Like Receptors Cascades