HEMC Protein (AA 1-306) (His tag)

Catalog No. ABIN1655523

Product Details

Target: HEMC (Porphobilinogen Deaminase (HEMC))

Protein Type: Recombinant

Protein Characteristics: AA 1-306

Origin: Acidithiobacillus ferrooxidans

Source: Yeast

Purification tag / Conjugate: This HEMC protein is labelled with His tag.

Application: ELISA

Sequence: MPHSLRIGTR ASPLAVWQAE HVRAALLRLH PGMAVEIITM TTSGDVLLDA PLHALGGKGL FVKEIEDALQ QRRVDVAVHS MKDVPALQPD GLEIVAIMAR EDVRDAFVSN TFLHPDALPE GARVGSSSLR RRAQLLERYP HLRVEDLRGN VATRLRRLDE GHYDAIILAA AGLKRLGLPD RITHLLDIDR SLPAVGQGAI GIEARSDDRR TRELLAPLAD ADTQNCVLAE RSMNQVLGGD CRLPVAALAR WQGPQMLLRG LVATPDGRRV LHAEAKGSDP VALGMAVAAA LVAQGAAEII EDVRNA

Specificity: Acidithiobacillus ferrooxidans (strain ATCC 53993) (Leptospirillum ferrooxidans (ATCC 53993))

Characteristics: Please inquire if you are interested in this recombinant protein expressed in E. coli, mammalien cells or by baculovirus infection. Be aware about differences in price and lead time.

Purity: > 90 %

Application Details

Comment:

The yeast protein expression system is the most economical and efficient eukaryotic system for secretion and intracellular expression. A protein expressed by the mammalian cell system is of very high-quality and close to the natural protein. But the low expression level, the high cost of medium and the culture conditions restrict the promotion of mammalian cell expression systems. The yeast protein expression system serve as a eukaryotic system integrate the advantages of the mammalian cell expression system. A protein expressed by yeast system could be modificated such as glycosylation, acylation, phosphorylation and so on to ensure the native protein conformation. It can be used to produce protein material with high added value that is very close to the natural protein. Our proteins produced by yeast expression system has been used as raw materials for downstream preparation of monoclonal antibodies.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: 0.2-2 mg/mL

Buffer: Tris-based buffer, 50 % glycerol

Handling Advice: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week

Storage: -20 °C

Storage Comment: Store at -20 °C, for extended storage, conserve at -20 °C or -80 °C.

Target Details

Target: HEMC (Porphobilinogen Deaminase (HEMC))

Abstract: HEMC Products

Synonyms: F8L15.10 Protein, F8L15_10 Protein, PORPHOBILINOGEN DEAMINASE Protein, hydroxymethylbilane synthase Protein, hydroxymethylbilane synthase Protein, HEMC Protein

Background: Recommended name: Porphobilinogen deaminase.
Short name= PBG.
EC= 2.5.1.61.
Alternative name(s): Hydroxymethylbilane synthase.
Short name= HMBS Pre-uroporphyrinogen synthase

UniProt: B5ERN6