PUS3 Protein (AA 1-442) (His tag)
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- Target See all PUS3 Proteins
- PUS3 (Pseudouridylate Synthase 3 (PUS3))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-442
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Origin
- Saccharomyces cerevisiae
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Source
- Yeast
- Purification tag / Conjugate
- This PUS3 protein is labelled with His tag.
- Application
- ELISA
- Sequence
- MSNFIRRLVG KMKAISTGTN AIVSKKDSIY ANWSKEQLIR RITELENANK PHSEKFQHIE DNKKRKISQE EVTRSKAKKA PKKFDFSKHN TRFIALRFAY LGWNYNGLAV QKEYTPLPTV EGTILEAMNK CKLVPSMVLQ DYKFSRCGRT DKGVSAMNQV ISLEVRSNLT DEEQRDPTND SREIPYVHVL NQLLPDDIRI SAVCLRPPPN FDARFSCVHR HYKYIFNGKN LNIEKMSKAA SYFVGERDFR NFCKLDGSKQ ITNFKRTIIS SKILPLSETF YCFDLVGSAF LWHQVRCMMA ILFLVGQSLE VPEIVLRLTD IEKTPQRPVY EMANDIPLLL YDCKFPEMDW QEPTVDDYKA IKFTTATEAL TLHYELKAAV CNIFKDVLPT ANTNNFSKTI INLGDGRGKV VGTYVKLEDR SVMEPVEVVN AKYSKKKNNK NK
- Specificity
- Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Bakers yeast)
- Characteristics
- Please inquire if you are interested in this recombinant protein expressed in E. coli, mammalien cells or by baculovirus infection. Be aware about differences in price and lead time.
- Purity
- > 90 %
- Top Product
- Discover our top product PUS3 Protein
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Pseudouridylate Synthase 3 (PUS3) (AA 1-481) protein (GST tag)
PUS3 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Pseudouridylate Synthase 3 (PUS3) (AA 2-481) protein (His tag)Crystallography grade PUS3 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
Pseudouridylate Synthase 3 (PUS3) (AA 2-481) protein (His tag)Crystallography grade PUS3 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
Pseudouridylate Synthase 3 (PUS3) protein (Myc-DYKDDDDK Tag)PUS3 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Comment
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The yeast protein expression system is the most economical and efficient eukaryotic system for secretion and intracellular expression. A protein expressed by the mammalian cell system is of very high-quality and close to the natural protein. But the low expression level, the high cost of medium and the culture conditions restrict the promotion of mammalian cell expression systems. The yeast protein expression system serve as a eukaryotic system integrate the advantages of the mammalian cell expression system. A protein expressed by yeast system could be modificated such as glycosylation, acylation, phosphorylation and so on to ensure the native protein conformation. It can be used to produce protein material with high added value that is very close to the natural protein. Our proteins produced by yeast expression system has been used as raw materials for downstream preparation of monoclonal antibodies.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Concentration
- 0.2-2 mg/mL
- Buffer
- Tris-based buffer, 50 % glycerol
- Handling Advice
- Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week
- Storage
- -20 °C
- Storage Comment
- Store at -20 °C, for extended storage, conserve at -20 °C or -80 °C.
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- Target
- PUS3 (Pseudouridylate Synthase 3 (PUS3))
- Alternative Name
- tRNA pseudouridine (38/39) synthase (PUS3 Products)
- Synonyms
- RGD1310757 Protein, 2610020J05Rik Protein, 5730412F04Rik Protein, pseudouridylate synthase 3 Protein, pseudouridine synthase 3 Protein, Pus3 Protein, PUS3 Protein
- Background
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Recommended name: tRNA pseudouridine(38/39) synthase.
EC= 5.4.99.45.
Alternative name(s): Depressed growth-rate protein DEG1 tRNA pseudouridine synthase 3 tRNA pseudouridylate synthase 3 tRNA-uridine isomerase 3 - UniProt
- P31115
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