V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) (Active) Protein

Details for Product No. ABIN1686509, Supplier: Log in to see
Protein Name
  • AKT2
  • pkbb
  • pkbbeta
  • pkbss
  • prkbb
  • rac-beta
  • v-akt2
  • 2410016A19Rik
  • AW554154
  • PKB
  • PKBbeta
  • HIHGHH
  • PKBB
  • PKBBETA
  • PRKBB
  • RAC-BETA
  • cb945
  • akt2-a
  • AKT serine/threonine kinase 2
  • v-akt murine thymoma viral oncogene homolog 2
  • thymoma viral proto-oncogene 2
  • v-akt murine thymoma viral oncogene homolog 2 L homeolog
  • AKT2
  • akt2
  • Akt2
  • akt2.L
Origin
Human
22
1
1
1
Source
Baculovirus infected Insect Cells
9
8
3
2
1
1
1
Protein Type
Recombinant
Biological Activity
Active
Application
Functional Studies (Func), SDS-PAGE (SDS), Western Blotting (WB)
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Purpose >90% Pure active Recombinant Akt2.
Characteristics Protein Source: Baculovirus (Sf9 insect cells)
Purity SDS-PAGE: ≥90 %
Background Akt2 or Protein Kinase B β (PKBβ) is a serine/threonine kinase that is a member of the Akt family. Akt2 like the other Akt members is activated in cells in response to diverse stimuli such as hormones, growth factors and extracellular matrix components and is involved in glucose metabolism, transcription, survival, cell proliferation, angiogenesis, and cell motility (1). The PI3K generates phosphatidylinositol-3, 4, 5-trisphosphate (PIP3), a lipid second messenger essential for the translocation of Akt2 to the plasma membrane where it is phosphorylated and activated by phosphoinositide-dependent kinase-1 (PDK-1) (2) and phosphoinositide-dependent kinase-2. Akt1 has numerous cellular substrates including proteins, which promote the inhibition of apoptosis such as the Forkhead transcription factors and the Bcl-2 family member Bad (3).
Alternate Names: Akt, RAC-alpha serine/threonine-protein kinase
Molecular Weight 58.0 kDa
Gene ID 208
UniProt P31751
Pathways PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, TLR Signaling, Cellular Glucan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Hepatitis C, VEGF Signaling
Application Notes Optimal working dilution should be determined by the investigator.
Comment

Activity Specification: 46 nmol phosphate incorporated into Akt/SGK substrate (RPRAATF) per minute per mg protein at 30°C for 15 minutes using a final concentration of 50 μM ATP and total of 0.83 μCi/μl P-32.

Restrictions For Research Use only
Format Liquid
Concentration 0.1 μg/μL
Buffer Recombinant proteins in storage buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25 % glycerol).
Preservative Dithiothreitol (DTT)
Precaution of Use This product contains dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Centrifuge the vial prior to opening.
Storage -80 °C
Storage Comment -80°C
Expiry Date 12 months
Supplier Images
SDS-PAGE (SDS) image for V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) (Active) protein (ABIN1686509) V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) (Active) protein
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