RAB5 Protein (His tag)

Catalog No. ABIN1686703

Product Details

Target: RAB5 (RAB5A) (RAB5A, Member RAS Oncogene Family (RAB5A))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This RAB5 protein is labelled with His tag.

Application: SDS-PAGE (SDS), Western Blotting (WB)

Sequence: MASRGATRPN GPNTGNKICQ FKLVLLGESA VGKSSLVLRF VKGQFHEFQE STIGAAFLTQ TVCLDDTTVK FEIWDTAGQE RYHSLAPMYY RGAQAAIVVY DITNEESFAR AKNWVKELQR QASPNIVIAL SGNKADLANK RAVDFQEAQS YADDNSLLFM ETSAKTSMNV NEIFMAIAKK LPKNEPQNPG ANSARGRGVD LTEPTQPTRN QCCSN

Specificity: ~26 kDa

Purification: Affinity Purified

Purity: >90%

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

This product has been certified >90% pure using SDS-PAGE analysis.

Restrictions: For Research Use only

Handling

Concentration: Lot specific

Buffer: 20 mM Tris/HCl pH 7.5, 0.45M NaCl, 10 % glycerol, 0.5 mM DTT

Storage: -20 °C

Target Details

Target: RAB5 (RAB5A) (RAB5A, Member RAS Oncogene Family (RAB5A))

Alternative Name: Rab5 (RAB5A Products)

Synonyms: RAB5 Protein, 2410015H04Rik Protein, AI663973 Protein, AU021172 Protein, nnyRab5a Protein, DDBDRAFT_0168594 Protein, DDBDRAFT_0229401 Protein, DDB_0168594 Protein, DDB_0229401 Protein, rab5 Protein, RAB5A Protein, Rab5a Protein, rab5al Protein, zgc:56644 Protein, fj38g08 Protein, hm:zehn1144 Protein, rab5a Protein, wu:fj38g08 Protein, RAB5A, member RAS oncogene family Protein, rab5A protein Protein, Rab5a, GTPase Protein, predicted protein Protein, Rab GTPase Protein, RAB5A, member RAS oncogene family L homeolog Protein, RAB5A, member RAS oncogene family, b Protein, RAB5A, member RAS oncogene family, a Protein, RAB5A Protein, Rab5a Protein, rab5A Protein, rab5a Protein, rab5a.L Protein, rab5ab Protein, rab5aa Protein

Background: Rab5 is a 24 kDa member of the Rab family of small guanosine triphosphatases (GTPases), Ras superfamily. Rab GTPases are central regulators of membrane trafficking in the eukaryotic cell. Their regulatory capacity depends on their ability to cycle between the GDP -bound inactive and GTP-bound active states. This conversion is regulated by GDP/GTP exchange factors (GEPs), GDP dissociation inhibitors (GDIs) and GTPase-activating proteins (GAPs) (1, 2). Activation of a Rab protein is coupled to its association with intracellular membranes, allowing it to recruit downstream effector proteins to the cytoplasmic surface of a subcellular compartment (3). Through these proteins, Rab GTPases regulate vesicle formation, actin- and tubulin-dependent vesicle movement, and membrane fusion(1). Rab proteins contain conserved regions involved in guanine-nucleotide binding, and hyper variable COHO-terminal domains with a cysteine motif implicated in subcellular targeting. Post-translational modification of the cysteine motif with one or two geranyl groups is essential for the membrane association and correct intracellular localization of Rab proteins(3). Each Rab shows a characteristic subcellular distribution (4). In particular, Rab5 is ubiquitously expressed in human tissues. It localizes mainly to early endosomes, but is also present on the plasma membrane. It regulates the fusion between endocytic vesicles and early endosomes, as well as the homotypic fusion between early endosomes (5). Among the proteins recruited by the GTP-bound active Rab5 are Rabaptin-5 and EEA1 (6). Anti-Rab5 may be used as an early endosome marker.

Molecular Weight: approx. 26 kDa

Gene ID: 5868

UniProt: P20339

Pathways: Smooth Muscle Cell Migration, Regulation of long-term Neuronal Synaptic Plasticity