CDC37 Protein (His tag)

Catalog No. ABIN1686724

Product Details

Target: CDC37 (Cell Division Cycle 37 Homolog (S. Cerevisiae) (CDC37))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This CDC37 protein is labelled with His tag.

Application: SDS-PAGE (SDS), Western Blotting (WB), Functional Studies (Func)

Sequence: MGHHHHHHMV DYSVWDHIEV SDDEDETHPN IDTASLFRWR HQARVERMEQ FQKEKEELDR GCRECKRKVA ECQRKLKELE VAEGGKAELE RLQAEAQQLR KEERSWEQKL EEMRKKEKSM PWNVDTLSKD GFSKSMVNTK PEKTEEDSEE VREQKHKTFV EKYEKQIKHF GMLRRWDDSQ KYLSDNVHLV CEETANYLVI WCIDLEVEEK CALMEQVAHQ TIVMQFILEL AKSLKVDPRA CFRQFFTKIK TADRQYMEGF NDELEAFKER VRGRAKLRIE KAMKEYEEEE RKKRLGPGGL DPVEVYESLP EELQKCFDVK DVQMLQDAIS KMDPTDAKYH MQRCIDSGLW VPNSKASEAK EGEEAGPGDP LLEAVPKTGD EKDVSV

Specificity: ~44.5 kDa

Characteristics: 4 µM SPR-304, when added to 2 µM SPR-300 (Aha1)-activated Hsp90 (2 µM, His-tagged Hsp90 beta) in 33 mM Hepes pH 7.2, 30 mM NaCl, 5 mM MgCl2, 1 mM DTT, 1.5 mM ATP in a 100 µL reaction at 37 degrees C, eliminated all Aha1-mediated ATPase stimulation as well as intrinsic Hsp90 ATPase activity. (This is an enzyme-linked ATP regeneration assay tracking loss of NADH absorbance at 340nm).

Purification: Affinity Purified

Purity: >90%

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

This product has been certified >90% pure using SDS PAGE analysis.

Restrictions: For Research Use only

Handling

Concentration: Lot specific

Buffer: 25 mM Tris buffer pH 7.2, 150 mM NaCl, 0.1DTT, 50 % glycerol

Storage: -20 °C

Target Details

Target: CDC37 (Cell Division Cycle 37 Homolog (S. Cerevisiae) (CDC37))

Alternative Name: CDC37 (CDC37 Products)

Synonyms: CG12019 Protein, CPDR-1 Protein, CPDR-2 Protein, DROCPDR Protein, Dmel\\CG12019 Protein, E(sev)3B Protein, cdc37 Protein, Afu4g10010 Protein, P50CDC37 Protein, p50 Protein, p50Cdc37 Protein, CG12019 gene product from transcript CG12019-RA Protein, cell division cycle 37 homolog Protein, Hsp90 co-chaperone Cdc37 Protein, Cdc37p Protein, cell division cycle 37 Protein, Cdc37 Protein, cdc37 Protein, AFUA_4G10010 Protein, CDC37 Protein

Background: HSP90 co-chaperone Cdc37 is a protein that is encoded by the CDC37 gene. It has been found to form complexes with HSP90 and a variety of protein kinases including CDK4, CDK6, SRC, RAF1, MOK and elF-2 alpha kinases. It is thought to play a critical role in directing HSP90 to its target kinases (1, 2). CDC37 is necessary for maintaining prostate tumor cell growth and represents a novel target in the exploration for multi-targeted therapies (3, 4).

Molecular Weight: Approx. 44.5 kDa

Gene ID: 11140

NCBI Accession: NP_008996

UniProt: Q16543