Proteoglycan 3 Protein (PRG3) (AA 18-225) (His tag)
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- Target See all Proteoglycan 3 (PRG3) Proteins
- Proteoglycan 3 (PRG3)
- Protein Type
- Recombinant
- Protein Characteristics
- AA 18-225
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Origin
- Human
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Source
- Human Cells
- Purification tag / Conjugate
- This Proteoglycan 3 protein is labelled with His tag.
- Purpose
- Recombinant Human Proteoglycan 3/PRG3 (C-6His)
- Sequence
- LHLENDAPHL ESLETQADLG QDLDSSKEQE RDLALTEEVI QAEGEEVKAS ACQDNFEDEE AMESDPAALD KDFQCPREED IVEVQGSPRC KTCRYLLVRT PKTFAEAQNV CSRCYGGNLV SIHDFNFNYR IQCCTSTVNQ AQVWIGGNLR GWFLWKRFCW TDGSHWNFAY WSPGQPGNGQ GSCVALCTKG GYWRRAQCDK QLPFVCSFVD HHHHHH
- Characteristics
- Recombinant Human Proteoglycan 3/PRG3 is produced by our mammalian expression system in human cells. The target protein is expressed with sequence (Leu18-Phe225) of Human PRG3 fused with a polyhistidine tag at the C-terminus.
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Sterility
- 0.2 μm filtered
- Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
- Top Product
- Discover our top product PRG3 Protein
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - Buffer
- Lyophilized from a 0.2 μm filtered solution of 20 mM PB,150 mM NaCl, pH 7.2.
- Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- Storage
- 4 °C/-20 °C/-80 °C
- Storage Comment
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Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months. - Expiry Date
- 3 months
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- Target
- Proteoglycan 3 (PRG3)
- Alternative Name
- Proteoglycan-3 (PRG3 Products)
- Synonyms
- MBP2 Protein, MBPH Protein, proteoglycan 3, pro eosinophil major basic protein 2 Protein, proteoglycan 3 Protein, PRG3 Protein, Prg3 Protein
- Sub Type
- Fusionprotein
- Background
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Proteoglycan 3, also known as Eosinophil major basic protein homolog, Prepro-major basic protein homolog, PRG3 and MBPH, contains one C-type lectin domain. Proteoglycans are a major component of the animal extracellular matrix. PRG3 localizes to the eosinophil secondary granule and is expressed in bone marrow, not detected in placenta. PRG3 has similar cytotoxic and cytostimulatory activities to PRG2/MBP. In vitro, PRG3 can stimulate neutrophil superoxide production and IL8 release, histamine and leukotriene C4 release from basophils.
Alternative Names: Proteoglycan 3, Eosinophil Major Basic Protein Homolog, Prepro-Major Basic Protein Homolog, Prepro-MBPH, PRG3, MBPH - Molecular Weight
- 24.6 kDa
- UniProt
- Q9Y2Y8
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