GFRA1 Protein (AA 25-429) (His tag)

Catalog No. ABIN1691698

Product Details

Target: GFRA1 (GDNF Family Receptor alpha 1 (GFRA1))

Protein Type: Recombinant

Protein Characteristics: AA 25-429

Origin: Human

Source: Human Cells

Purification tag / Conjugate: This GFRA1 protein is labelled with His tag.

Purpose: Recombinant Human GDNF Family Receptor α-1/GFRA1 (C-Fc)

Sequence: DRLDCVKASD QCLKEQSCST KYRTLRQCVA GKETNFSLAS GLEAKDECRS AMEALKQKSL YNCRCKRGMK KEKNCLRIYW SMYQSLQGND LLEDSPYEPV NSRLSDIFRV VPFISVEHIP KGNNCLDAAK ACNLDDICKK YRSAYITPCT TSVSNDVCNR RKCHKALRQF FDKVPAKHSY GMLFCSCRDI ACTERRRQTI VPVCSYEERE KPNCLNLQDS CKTNYICRSR LADFFTNCQP ESRSVSSCLK ENYADCLLAY SGLIGTVMTP NYIDSSSLSV APWCDCSNSG NDLEECLKFL NFFKDNTCLK NAIQAFGNGS DVTVWQPAFP VQTTTATTTT ALRVKNKPLG PAGSENEIPT HVLPPCANLQ AQKLKSNVSG NTHLCISNGN YEKEGLGASS HITTKVDDIE GRMDEPKSCD KTHTCPPCPA PELLGGPSVF LFPPKPKDTL MISRTPEVTC VVVDVSHEDP EVKFNWYVDG VEVHNAKTKP REEQYNSTYR VVSVLTVLHQ DWLNGKEYKC KVSNKALPAP IEKTISKAKG QPREPQVYTL PPSREEMTKN QVSLTCLVKG FYPSDIAVEW ESNGQPENNY KTTPPVLDSD GSFFLYSKLT VDKSRWQQGN VFSCSVMHEA LHNHYTQKSL SLSPGK

Characteristics: Recombinant Human GDNF Family Receptor alpha-1/GFRA1 is produced with our mammalian expression system in human cells. The target protein is expressed with sequence (Asp25-Lys429) of Human GFRA1 fused with a polyhistidine tag at the C-terminus.

Purity: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 5 months

Target Details

Target: GFRA1 (GDNF Family Receptor alpha 1 (GFRA1))

Alternative Name: GFRA1 (GFRA1 Products)

Synonyms: gdnfr Protein, ret1l Protein, retl1 Protein, trnr1 Protein, gdnfra Protein, MGC122616 Protein, gfr-alpha-1 Protein, GDNFR Protein, GDNFRA Protein, GFR-ALPHA-1 Protein, RET1L Protein, RETL1 Protein, TRNR1 Protein, GDNFRALPHA Protein, GFRA1 Protein, AU042498 Protein, gfralpha1a Protein, zgc:110129 Protein, GDNF family receptor alpha 1 Protein, glial cell line derived neurotrophic factor family receptor alpha 1 Protein, gdnf family receptor alpha 1a Protein, gfra1 Protein, GFRA1 Protein, Gfra1 Protein, gfra1a Protein

Sub Type: Fusionprotein

Background: Glial Cell Line-Derived Neurotrophic Factor Family Receptor alpha-1 (GDNFRalpha1) is a glycosylphosphatidylinositol (GPI) linked cell surface protein belonging to GDNF-family receptor alpha subtype which consists of at least four members. GFRalpha1and GFRalpha2 are the cognate co-receptor for the neurotrophic factor neurturin mediating the NRTN-induced autophosphorylation and activation of the RET tyrosine kinase receptor. Soluble GFRalphas released enzymatically from the cell surface by phosphatidylinositol phospholipase C, as well as recombinantly produced soluble GFRalpha1, can also bind with high affinity to GDNF and trigger the activation of Ret tyrosine kinase. Human GFRalpha1 shares 93% amino acid identity with mouse GFRalpha1.The expression of the various GFRalphas are differentially regulated in the central and peripheral nervous system, suggesting complementary roles for the GFRalphas in mediating the activities of the GDNF family of neurotrophic factors.
Alternative Names: GDNF Family Receptor Alpha-1, GDNF Receptor Alpha-1, GDNFR-Alpha-1, GFR-Alpha-1, RET Ligand 1, TGF-Beta-Related Neurotrophic Factor Receptor 1, GFRA1, GDNFRA, RETL1, TRNR1

Molecular Weight: 72.3 kDa

UniProt: P56159