Iba1 Protein (AA 2-147) (His tag)
-
- Target See all Iba1 (IBA1) Proteins
- Iba1 (IBA1) (Ionized Calcium-binding Adapter Molecule 1 (IBA1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 2-147
-
Origin
- Human
-
Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This Iba1 protein is labelled with His tag.
- Purpose
- Recombinant Human Allograft Inflammatory Factor 1/AIF1 (C-6His)
- Sequence
- SQTRDLQGGK AFGLLKAQQE ERLDEINKQF LDDPKYSSDE DLPSKLEGFK EKYMEFDLNG NGDIDIMSLK RMLEKLGVPK THLELKKLIG EVSSGSGETF SYPDFLRMML GKRSAILKMI LMYEEKAREK EKPTGPPAKK AISELPLEHH HHHH
- Characteristics
- Recombinant Human Allograft Inflammatory Factor 1/AIF1 is produced with our E. coli expression system. The target protein is expressed with sequence (Ser2-Pro147) of Human AIF1 fused with a 6His tag at the C-terminus.
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Sterility
- 0.2 μm filtered
- Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
- Top Product
- Discover our top product IBA1 Protein
-
-
- Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Reconstitution
-
It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - Buffer
- Lyophilized from a 0.2 μm filtered solution of 20 mM PB,150 mM NaCl, pH 7.4.
- Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- Storage
- 4 °C/-20 °C/-80 °C
- Storage Comment
-
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months. - Expiry Date
- 3 months
-
- Target
- Iba1 (IBA1) (Ionized Calcium-binding Adapter Molecule 1 (IBA1))
- Alternative Name
- AIF1 (IBA1 Products)
- Synonyms
- AIF-1 Protein, IBA1 Protein, IRT-1 Protein, IRT1 Protein, AI607846 Protein, D17H6S50E Protein, G1 Protein, Iba1 Protein, Bart1 Protein, iba1 Protein, mrf-1 Protein, fa04b11 Protein, wu:fa04b11 Protein, AIF Protein, AIF1 Protein, aif1 Protein, allograft inflammatory factor 1 Protein, AIF1 Protein, Aif1 Protein, aif1 Protein
- Sub Type
- Fusionprotein
- Background
-
Allograft Inflammatory Factor 1 (AIF1) contains two EF-hand domains and exists as a homodimer. AIF1 can be detected in T-lymphocytes and peripheral blood mononuclear cells. AIF1 functions as actin-binding protein that enhances membrane ruffling and RAC activation and can enhance the actin-bundling activity of LCP1. In addition, AIF1 plays a role in RAC signaling and in phagocytosis and may also in macrophage activation and function. AIF1 promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes and plays a role in vascular inflammation.
Alternative Names: Allograft Inflammatory Factor 1, AIF-1, Ionized Calcium-Binding Adapter Molecule 1, Protein G1, AIF1, G1, IBA1 - Molecular Weight
- 17.7 kDa
- UniProt
- P55008
- Pathways
- Smooth Muscle Cell Migration
-