Deoxyuridine Triphosphatase (DUT) (AA 1-164) Protein

Catalog No. ABIN1692033

Product Details

Target: Deoxyuridine Triphosphatase (DUT)

Protein Type: Recombinant

Protein Characteristics: AA 1-164

Origin: Human

Source: Escherichia coli (E. coli)

Purpose: Recombinant Human dUTP Pyrophosphatase/dUTPase

Sequence: MPCSEETPAI SPSKRARPAE VGGMQLRFAR LSEHATAPTR GSARAAGYDL YSAYDYTIPP MEKAVVKTDI QIALPSGCYG RVAPRSGLAA KHFIDVGAGV IDEDYRGNVG VVLFNFGKEK FEVKKGDRIA QLICERIFYP EIEEVQALDD TERGSGGFGS TGKN

Characteristics: Recombinant Human Deoxyuridine 5'-Triphosphate Nucleotidohydrolase Mitochondrial/DUT is produced with our E. coli expression system. The target protein is expressed with sequence (Met1-Asn164) of Human Dutpase.

Purity: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Supplied as a 0.2 μm filtered solution of 20 mM PB,150 mM NaCl, pH 7.4.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: -80 °C

Storage Comment: Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.

Expiry Date: 6 months

Target Details

Target: Deoxyuridine Triphosphatase (DUT)

Alternative Name: Dutpase (DUT Products)

Synonyms: BcDNA:LD08534 Protein, CG4584 Protein, Dmel\\CG4584 Protein, LD08534 Protein, UTPase Protein, anon-SAGE:Wang-077 Protein, dUTPase Protein, Tb07.27E10.390 Protein, dutpase Protein, 5031412I06Rik Protein, 5133400F09Rik Protein, D2Bwg0749e Protein, Dutp Protein, PIP4 Protein, deoxyuridine triphosphatase Protein, Deoxyuridine triphosphatase Protein, dUTPase; ORF54; similar to EBV BLLF3, CMV UL72, HSV UL50 Protein, involved in nucleotide metabolism Protein, tegument protein Protein, dUTPase Protein, deoxyuridine triphosphatase L homeolog Protein, DUT Protein, dUTPase Protein, P18 Protein, AlHV1gp51 Protein, UL50 Protein, HVT058 Protein, ORF9 Protein, ORF8 Protein, U45 Protein, GAMMAHV.ORF54 Protein, Tc00.1047053509151.130 Protein, Tc00.1047053508175.160 Protein, Tb927.7.5160 Protein, dut.L Protein, Dut Protein

Target Type: Viral Protein

Background: Deoxyuridine 5'-Triphosphate Nucleotidohydrolase Mitochondrial (dUTPase) belongs to the dUTPase family. dUTPase exits as a homotrimer and is involved in nucleotide metabolism. dUTPase produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA. The dUTPase increase in PCR product yield, length and fidelity enables further down-stream applications. These effects make dUTPase useful in PCR fidelity and yield-sensitive applications. dUTPase is specific for dUTP and is critical for the fidelity of DNA replication and repair.
Alternative Names: Deoxyuridine 5'-Triphosphate Nucleotidohydrolase Mitochondrial, dUTPase, dUTP Pyrophosphatase, DUT

Molecular Weight: 17.7 kDa