RPE Protein (AA 1-228) (His tag)
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- Target See all RPE Proteins
- RPE (Ribulose-5-Phosphate-3-Epimerase (RPE))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-228
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This RPE protein is labelled with His tag.
- Purpose
- Recombinant Human Ribulose-Phosphate 3-Epimerase/RPE (C-6His)
- Sequence
- MASGCKIGPS ILNSDLANLG AECLRMLDSG ADYLHLDVMD GHFVPNITFG HPVVESLRKQ LGQDPFFDMH MMVSKPEQWV KPMAVAGANQ YTFHLEATEN PGALIKDIRE NGMKVGLAIK PGTSVEYLAP WANQIDMALV MTVEPGFGGQ KFMEDMMPKV HWLRTQFPSL DIEVDGGVGP DTVHKCAEAG ANMIVSGSAI MRSEDPRSVI NLLRNVCSEA AQKRSLDRVD HHHHHH
- Characteristics
- Recombinant Human Ribulose-Phosphate 3-Epimerase/RPE is produced with our E. coli expression system. The target protein is expressed with sequence (Met1-Arg228) of Human RPE fused with a 6His tag at the C-terminus.
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Sterility
- 0.2 μm filtered
- Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
- Top Product
- Discover our top product RPE Protein
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- Restrictions
- For Research Use only
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- Format
- Liquid
- Reconstitution
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - Buffer
- Supplied as a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 6.2.
- Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- Storage
- -80 °C
- Storage Comment
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Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles. - Expiry Date
- 6 months
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- Target
- RPE (Ribulose-5-Phosphate-3-Epimerase (RPE))
- Alternative Name
- RPE (RPE Products)
- Synonyms
- wu:fa07h08 Protein, wu:fb93d11 Protein, wu:fc20b11 Protein, ik:tdsubc_2c8 Protein, xx:tdsubc_2c8 Protein, RPE2-1 Protein, 2810429B02Rik Protein, 5730518J08Rik Protein, ribulose-5-phosphate-3-epimerase Protein, rpe Protein, RPE Protein, Rpe Protein
- Sub Type
- Fusionprotein
- Background
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Ribulose-Phosphate 3-Epimerase (RPE) is a member of the Ribulose-Phosphate 3-Epimerase family. RPE exists as a homodimer and catalyzes the reversible epimerization of D-ribulose 5-phosphate to D-xylulose 5-phosphate. RPE binds one divalent metal cation per subunit and contains tightly bound Fe2+ when produced in E. coli, but the physiological cofactor may be Co2+, Mn2+ or Zn2+. It has been shown that RPE participates in 3 metabolic pathways: pentose phosphate pathway, pentose and glucuronate interconversions, and carbon fixation.
Alternative Names: Ribulose-Phosphate 3-Epimerase, Ribulose-5-Phosphate-3-Epimerase, RPE, HUSSY-17 - Molecular Weight
- 25.9 kDa
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