HDGF Protein (AA 1-100) (His tag)

Catalog No. ABIN1692053

Product Details

Target: HDGF (Hepatoma-Derived Growth Factor (HDGF))

Protein Type: Recombinant

Protein Characteristics: AA 1-100

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This HDGF protein is labelled with His tag.

Purpose: Recombinant Human Hepatoma-Derived Growth Factor/HDGF (C-6His)

Sequence: MSRSNRQKEY KCGDLVFAKM KGYPHWPARI DEMPEAAVKS TANKYQVFFF GTHETAFLGP KDLFPYEESK EKFGKPNKRK GFSEGLWEIE NNPTVKASGY LEHHHHHH

Characteristics: Recombinant Human Hepatoma-Derived Growth Factor/HDGF is produced with our E. coli expression system. The target protein is expressed with sequence (Met1-Tyr100) of Human HDGF fused with a 6His tag at the C-terminus.

Purity: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM Tris, 1 mM DTT, 1 mM EDTA, pH 7.5.

Preservative: Dithiothreitol (DTT)

Precaution of Use: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 3 months

Target Details

Target: HDGF (Hepatoma-Derived Growth Factor (HDGF))

Alternative Name: Hepatoma-Derived Growth Factor/HDGF (HDGF Products)

Synonyms: HMG1L2 Protein, hmg1l2 Protein, HDGF Protein, DKFZp459H185 Protein, AI118077 Protein, D3Ertd299e Protein, heparin binding growth factor Protein, hepatoma-derived growth factor Protein, hepatoma-derived growth factor (high-mobility group protein 1-like) Protein, heparin binding growth factor L homeolog Protein, hepatoma-derived growth factor-like Protein, HDGF Protein, hdgf Protein, Hdgf Protein, hdgf.L Protein, LOC100357981 Protein

Sub Type: Fusionprotein

Background: Hepatoma-Derived Growth Factor is a original member of the HDGF family. HDGF is a cytoplasmic protein and contains one PWWP domain. HDGF expression levels are high in the nucleus and cytoplasm of smooth muscle and endothelial cells. HDGF has proliferative, angiogenic and neurotrophic activity. HDGF was initially characterized as a secreted mitogen from the Huh-7 human hepatoma cell line. As a heparin-binding protein, which is highly expressed in tumor cells where it stimulates proliferation. HDGF has mitogenic activity for fibroblasts and acts as a transcriptional repressor. It has been shown that HDGF is linked with tumorigenesis and the growth of cancer.
Alternative Names: Hepatoma-Derived Growth Factor, HDGF, High Mobility Group Protein 1-Kike 2, HMG-1L2, HDGF, HMG1L2

Molecular Weight: 12.6 kDa

UniProt: P51858

Pathways: ER-Nucleus Signaling